A New Approach to the Characterization of Sulfated and Sialyl Lewis-Type Glycosphingolipids Using Positive-Ion FABMS, ESIMS, and CID-MS/MS
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概要
- 論文の詳細を見る
Positive-ion fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry have been used in the structural characterization of sulfated and sialyl Lewis (Le)-type glycosphingolipids. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of acidic glycosphingolipids allowed identification of the sulfated and sialyl sugars, and provided information on the sugar chain sequences. The positive-ion tandem FABMS of sulfated Le-type glycosphingolipids gave the daughter ions produced by the elimination of (fucose-H2O) unit from the fragment ions containing the non-reducing end. On the other hand, the CID-MS/MS of sialyl analogs showed the characteristic ions derived from the loss of (N-acetylneuraminic acid-H2O) unit, in addition to the elimination of (fucose-H2O) unit. The ESI CID-MS/MS of multiple-charged ions provided even more detailed structural information, and some of the daughter ions useful for structural studies appeared in the higher mass region than the precursor owing to a lower charge-state. These methodologies can be applied to the structural analyses of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated and sialyl analogs.
- 日本質量分析学会の論文
- 1996-04-20
著者
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Ogawa Tomoya
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Ogawa Tomoya
Laboratory Of Synthetic Cellulary Chemistry The Institute Of Physical And Chemical Research (riken)
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OHASHI Yoko
Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R
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II Tadashi
Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R
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NAGAI Yoshitaka
Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R
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Nagai Y
Glycobioloyg Research Frontier Research Program The Institute Of Physical And Chemical Research (rik
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Nagai Y
Mitsubishi Kasei Institute Of Life Sciences
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Ii Tadashi
Glycobiology Research Froniter Research Program The Institute Of Physical And Chemical Research (rik
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Ohashi Y
Niigata Univ. School Of Medicine Niigata
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Ohashi Yoko
Glycobiology Research Frontier Research Program The Institute Of Physical And Chemical Research (rik
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Ogawa T
Osaka Univ. Dental School Osaka
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Ogawa Tomoya
Laboratory Of Cellular Biochemistry Animal Resource Science/veterinary Medical Science The Universit
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Ogawa Tomoya
Laboratory for Physics and Technology of Crystals, 2-9-21-604 Shin-sayama, Sayama, Saitama 350-1331, Japan
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