Nucleotide Sequence of eCG α-Subunit cDNA and Its Expression in the Equine Placenta
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概要
- 論文の詳細を見る
Equine chorionic gonadotropin (eCG) is a member of the glycoprotein family, which includes lutropin (LH), follitropin (FSH), and thyrotropin (TSH), all consisting of two noncovalently linked a-and β-subunits. In the present study, we determined the full length of the equine α-subunit cDNA sequence. mRNA was extracted from equine placenta on day 70 of gestation. The nucleotide sequence of the eCG a-subunit cDNA was amplified by PCR using mixed primers designed on the basis of the nucleotide sequence of human, rat and mouse a-subunits. Analysis of the nucleotide sequence revealed that the a-subunit amplified contained 387 bp which included the signal peptide (24 amino acids containing 4 amino acids expected) and the first 3 amino acid residues. When the expression of both subunit mRNAs in the equine placenta was compared by Northern blotting, the expression of the β-subunit mRNA was relatively greater than that of the a-subunit.
- 日本繁殖生物学会の論文
著者
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Min Kwan-Sik
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Shiota Kunio
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Ogawa Tomoya
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Shiota Kunio
Laboratory Of Cellular Biochemistry Animal Resource Science/veterinary Medical Sciences Graduate Sch
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Min Kwan-sik
Laboratory Of Cellular Biochemistry Veterinary Medical Science/animal Resource Science The Universit
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Min Kwan-sik
Cellular Biochemistry Veterinary Medical Science/animal Resource Science University Of Tokyo
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MIYAZAWA Kiyoshi
Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine
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Miyazawa Kiyoshi
Laboratory Of Theriogenology Division Of Veterinary Medicine Faculty Of Applied Biological Sciences
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Ogawa Tomoya
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers
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Min Kwan-sik
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences Graduate Sc
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SHINOZAKI Mikihiko
Laboratories of Cellular Biochemistry
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NISHIMURA Ryohei
Surgery, Veterinary Medical Science/Animal Resource Science, The University
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SASAKI Nobuo
Surgery, Veterinary Medical Science/Animal Resource Science, The University
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Ogawa Tomoya
Laboratory Of Cellular Biochemistry Animal Resource Science/veterinary Medical Science The Universit
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