Release of Anterior Pituitary Hormones by Trypsin-pretreated Anterior Pituitary Cells
スポンサーリンク
概要
- 論文の詳細を見る
Hormone-secreting cells in the anterior pituitary (AP) gland can be categorized into two groups with respect to spontaneous release. One line of evidence has suggested relatively high spontaneous release of GH and PRL compared to other hormones. We previously reported that brief pretreatment of AP cells with trypsin severely decreased the spontaneous release of GH and PRL, but increased that of ACTH and TSH, and had no apparent effect on that of LH and FSH. The cell-type specific alteration of spontaneous release indicates involvement of trypsin-sensitive-membrane-proteins (TSMPs) in the mechanism of hormone release in the AP gland. In this study, we examined secretagogue- and high-K+ stimulated release of AP hormones from trypsin-pretreated cells by using a superfusion system. TRH and high K+ did not provoke increased PRL release. Each of the secretagogues and high K+ caused a corresponding elevation of other hormones, i.e., TSH, ACTH, LH, and FSH, suggesting that the TRH receptor and voltage-sensitive Ca2+ channel remained functional. In addition, GRF and high K+ did not result in a corresponding increase in GH release. We concluded that the GH/PRL cell lineage has distinct secretory mechanisms including TSMPs.
- 日本繁殖生物学会の論文
著者
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Shiota Kunio
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Ogawa Tomoya
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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TAKAMORI Shigeo
Laboratory of Cellular Biochemistry, Department of Veterinary Medical Science/Animal Resource Scienc
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