マウスノロウイルスS7株に対する特異抗体を検出するための間接ELISA法および間接蛍光抗体法
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概要
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To evaluate murine norovirus (MNV) infection in laboratory mice, we attempted to develop an enzyme-linked immunosorbent assay (ELISA) system and an indirect immunofluorescent antibody (IFA) assay for detecting the anti-MNV-S7 antibody in mice. MNV-S7, which was isolated in Japan, was used in both assays. The antigen for ELISA was prepared by ultracentrifugation of culture supernatants of RAW 264 cells infected with MNV-S7. Positive sera were obtained from 6-week-old, female C57BL/6JJcl mice inoculated orally with MNV-S7. IFA against infected RAW 264 cells was able to discriminate positive sera from negative sera. Indirect ELISA was performed using 96-well ELISA plates coated with formalin-treated MNV-S7 antigen. In this ELISA system, mouse sera obtained 2 weeks after infection or later showed significantly high OD values and were judged positive. An equal level of anti-MNV-S7 antibody response was observed in BALB/cAJcl, C57BL/6JJcl, DBA/2JJcl, and Jcl:ICR mice; whereas, C3H/HeJJcl mice demonstrated slightly lower antibody production 4 weeks after infection. We also used this ELISA system to evaluate 77 murine serum samples obtained from 15 conventional mouse rooms in research facilities in Japan and found that approximately half of the serum samples contained antibody to MNV-S7. We found that some serum samples were negative for antibodies to mouse hepatitis virus and Mycoplasma pulmonis but positive for antibody to MNV-S7. The results suggest that the MNV infection is more prevalent than other infections such as mouse hepatitis virus and Mycoplasma pulmonis in conventional mouse colonies in Japan, as is the case in other areas of the world.
- 社団法人 日本実験動物学会の論文
- 2010-01-01
著者
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吉川 泰弘
東京大学 大学院農学生命科学研究科実験動物学教室
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北川 洋大
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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遠矢 幸伸
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The Univer
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池 郁生
Experimental Animal Division, RIKEN BioResource Center
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KAJITA Ayako
Experimental Animal Division, RIKEN BioResource Center
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PARK Sang-Jin
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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ISHII Yoshiyuki
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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KYUWA Shigeru
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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YOSHIKAWA Yasuhiro
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Ishii Yoshiyuki
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Ishii Yoshiyuki
東京大学 農生命科研究
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Ishii Yoshiyuki
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Yoshikawa Yasuhiro
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Kyuwa S
Univ. Tokyo Tokyo Jpn
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Kyuwa Shigeru
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Yoshikawa Y
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Yoshikawa Yasuhiro
Department Of Biomedical Science Graduate School Of Agricultural And Life Science University Of Toky
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Ishii Yoshiyuki
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kyuwa Shigeru
Department Of Animal Pathology Institute Of Medical Science University Of Tokyo
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吉川 泰弘
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Kyuwa Shigeru
Center For Experimental Medicine Institute Of Medical Science The University Of Tokyo
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KITAGAWA Yota
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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TOHYA Yukinobu
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The Univer
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IKE Fumio
Experimental Animal Division, RIKEN BioResource Center
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Yoshikawa Yasuhiro
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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吉川 泰弘
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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Tohya Yukinobu
Department Of Veterinary Microbiology Graduate School Of Agricultural And Life Sciences The Universi
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Kitagawa Yota
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Ike Fumio
Experimental Animal Division Riken Bioresource Center
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Park Sang-jin
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Kajita Ayako
Experimental Animal Division Riken Bioresource Center
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Tohya Yukinobu
Department Of Veterinary Microbiology Graduate School Of Agricultural And Life Sciences The Universi
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Ike Fumio
Experimental Animal Div. Riken Bioresource Center
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