Production of a Monoclonal Antibody Reacted Broadly with Feline Calicivirus Field Isolates.
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概要
- 論文の詳細を見る
A monoclonal antibody (MAb) reactive with 36 field isolates and 2 laboratory strains of feline calicivirus (FCV) was produced by immunizing mice with the mixture of FCVs. The MAb (4D7) reacted with FCVs in an enzyme-linked immunosorbent assay (ELISA), but had no neutralizing activity against the F4 strain of FCV. MAb 8G1, previously produced against the FCV F4 strain, also reacted in ELISA with all FCVs used in the present study. However, the epitopes recognized by 4D7 and 8G1 were different. Using these two MAbs and a polyclonal rabbit antibody, we attempted to develop a sandwich ELISA for detection of FCV antigen. The combination of 4D7 and the polyclonal rabbit IgG was most sensitive. Using this system, all the field isolates of FCV cultured in vitro were detected. However, among the 36 swab samples, from which FCV was isolated, 4 were negative.
- 公益社団法人 日本獣医学会の論文
著者
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Tohya Yukinobu
Department Of Veterinary Microbiology Graduate School Of Agricultural And Life Sciences The Universi
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SUGII Shunji
Department of Serology and Immunology, School of Medical Technology, Kitasato University
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TAJIMA Tomoko
Department of Infectious Diseases Control, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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TOHYA Yukinobu
Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University
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YOSHIZAKI Sachiko
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University
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NAKATA Erika
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University
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ISHIGURO Shinryo
Central Laboratories, Kyoritsu Shoji Company
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FUJIKAWA Yuji
Central Laboratories, Kyoritsu Shoji Company
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SUGII Shunji
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University
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