Green Fluorescent Protein Gene Insertion of Sendai Virus Infection in Nude Mice: Possibility as an Infection Tracer.

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概要

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• The green fluorescent protein (GFP) marker from jellyfish Aequorea victoria is considered to have potential use in the study of host-pathogen relationships, by tracing infections in living cells, organs and animals. We compared the pathogenicity of Sendai virus with an inserted GFP gene (GFP-SeV) with that of its wild-type (Wt-SeV) to determine the usefulness of the recombinant virus in long-term infection of BALB/c nude (nu/nu) mice. The results indicated that the presence of GFP in infected cells could be analyzed easily and sensitively. GFP helped in identifying and in understanding the cellular sites of viral replication in vitro and in vivo. However, the GFP insertion into the Wt-SeV genome, led to decreased pathogenicity, altering the in vivo viral kinetics.

• 公益社団法人 日本獣医学会の論文

著者

• Tohya Yukinobu

  Department Of Veterinary Microbiology Graduate School Of Agricultural And Life Sciences The Universi

• Tateyama Susumu

  Department Of Veterinary Pathology Faculty Of Agriculture Miyazaki University

• Kato Atsushi

  Department Of Biological Science Graduate School Of Life And Environment Science Shimane University

• Yamaguchi Ryoji

  Department Of Material System Engineering Faculty Of Technology Tokyo University Of Agriculture And Technology

• Ratih AGUNGPRIYONO

  Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Nishi 1-1, Gakuen Kibana Dai, Miyazaki 889-2155, Japan

• NAGAI Yoshiyuki

  AIDS Research Center, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan

• ASAKAWA Makoto

  DNAVEC Research Institute, 1-25-11 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

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