The Region-Specific Functions of Two Ubiquitin C-Terminal Hydrolase Isozymes along the Epididymis
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概要
- 論文の詳細を見る
We previously showed that gad mice, which are deficient for ubiquitin C-terminal hydrolase L1 (UCH-L1), have a significantly increased number of defective spermatozoa, suggesting that UCH-L1 functions in sperm quality control during epididymal maturation. The epididymis is the site of spermatozoa maturation, transport and storage. Region-specific functions along the epididymis are essential for establishing the environment required for sperm maturation. We analyzed the region-specific expression of UCH-L1 and UCH-L3 along the epididymis, and also assessed the levels of ubiquitin, which has specificity for UCH-L1. In wild-type mice, western blot analysis demonstrated a high level of UCH-L1 expression in the caput epididymis, consistent with ubiquitin expression, whereas UCH-L3 expression was high in the cauda epididymis. We also investigated the function of UCH-L1 and UCH-L3 in epididymal apoptosis induced by efferent duct ligation. The caput epididymides of gad mice were resistant to apoptotic stress induced by efferent duct ligation, whereas Uchl3 knockout mice showed a marked increase in apoptotic cells following ligation. In conclusion, the response of gad and Uchl3 knockout mice to androgen withdrawal suggests a reciprocal function of the two UCH enzymes in the caput epididymis.
- 社団法人 日本実験動物学会の論文
- 2006-01-01
著者
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吉川 泰弘
東京大学 大学院農学生命科学研究科実験動物学教室
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YOSHIKAWA Yasuhiro
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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SEKIGUCHI Satoshi
Department of Veterinary Pathology, Nippon Veterinary and Animal Science University
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KWON Jungkee
Department of Biomedical Science Graduate School of Agricultural and Life Sciences, The University o
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WADA Keiji
Department of Degenerative Neurological Diseases, National Institute of Neuroscience National Center
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Setsuie Rieko
Department Of Animal Models For Human Disease National Institute Of Neuroscience National Center Of
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Yoshikawa Yasuhiro
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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関口 智嗣
産業技術総合研究所グリッド研究センター
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WANG Yu-Lai
Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Cente
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Yoshikawa Y
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Yoshikawa Yasuhiro
Department Of Biomedical Science Graduate School Of Agricultural And Life Science University Of Toky
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Kwon Jungkee
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Kwon Jungkee
Department Of Laboratory Animal Medicine College Of Veterinary Medicine Chonbuk National University
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吉川 泰弘
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Wang Yu-lai
Department Of Degenerative Neurological Diseases National Institute Of Neuroscience National Center
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Wada Keiji
Department Of Biological Science Faculty Of Science Nara Women's University
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Yoshikawa Yasuhiro
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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吉川 泰弘
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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Wada K
Department Of Degenerative Neurological Diseases National Institute Of Neuroscience National Center
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吉川 泰弘
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Sekiguchi Satoshi
Department Of Chemistry Graduate School Of Science Tohoku University
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Wada Keiji
Department Of Biological Science Faculty Of Science
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