Protective Effects of Probucol Treatment on Pancreatic β-cell Function of SZ-induced Diabetic APA Hamsters
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概要
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To clarify whether oxidative stress is involved in the pathogenesis of islet lesions of diabetic animals, the effects of probucol (PB), an antioxidant and anti-hyperlipidemia agent, on the islets in streptozotocin (SZ)-induced diabetic APA hamsters in the acute and chronic phases of diabetes were examined. The control (CB group) and diabetic (SZ group) hamsters were treated with PB (1% in the diet) for 4 weeks from several days after SZ injection as the acute diabetic group, or 8 weeks from 6 weeks after SZ injection as the chronic diabetic group. Glucose tolerance test revealed that PB treatment decreased the high serum glucose level after glucose injection in the diabetic APA hamsters in the acute diabetic phase. Immunohistochemistry revealed that PB treatment significantly increased the percentage of the insulin positive area in the diabetic hamsters pancreata in both the acute and chronic phases. In addition, 4-hydroxy-2-nonenal (4HNE; an oxidative stress marker) positive cells were slightly reduced by PB treatment in the acute diabetic phase. Double-immunostaining for insulin and PCNA (proliferating cell nuclear antigen) revealed that elevation of the percentage of insulin and PCNA double-positive cells against insulin-positive cells was seen in the islets of PB-treated diabetic hamsters, but the difference was not significant compared with untreated diabetic hamsters (p=0.07). In semi-quantitative RT-PCR, the expression of two genes, Reg (Regenerating gene) and INGAP (islet neogenesis associated protein), in the diabetic APA hamsters was significantly increased compared to the control groups in both diabetic phases. PB treatment significantly reduced Reg expression in the chronic diabetic phase. These data suggest that PB treatment in SZ-injected diabetic hamsters partially restored β-cell function through acting as an antioxidant and induced higher expression of Reg and INGAP genes in the pancreas of hamsters.
- 社団法人 日本実験動物学会の論文
- 2003-07-01
著者
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吉川 泰弘
東京大学 大学院農学生命科学研究科実験動物学教室
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ISHII Yoshiyuki
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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KYUWA Shigeru
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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YOSHIKAWA Yasuhiro
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Ishii Yoshiyuki
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Ishii Yoshiyuki
東京大学 農生命科研究
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TAKATORI Atsushi
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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OHTA Etsuko
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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INENAGA Toshiaki
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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HORIUCHI Keiko
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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ITAGAKI Shin-ichi
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Ishii Y
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Ishii Yoshiyuki
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Yoshikawa Yasuhiro
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Kyuwa S
Univ. Tokyo Tokyo Jpn
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Kyuwa Shigeru
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Yoshikawa Y
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Yoshikawa Yasuhiro
Department Of Biomedical Science Graduate School Of Agricultural And Life Science University Of Toky
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Ohta Etsuko
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Ishii Yoshiyuki
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Horiuchi Keiko
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Kawamura S
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Kyuwa Shigeru
Department Of Animal Pathology Institute Of Medical Science University Of Tokyo
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吉川 泰弘
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Takatori Atsushi
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Inenaga Toshiaki
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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板垣 慎一
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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Itagaki Shin-ichi
Department Of Biomedical Science The University Of Tokyo
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Kyuwa Shigeru
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences University Of Tok
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Kyuwa Shigeru
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Kyuwa Shigeru
Center For Experimental Medicine Institute Of Medical Science The University Of Tokyo
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Yoshikawa Yasuhiro
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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吉川 泰弘
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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Itagaki Shin-ichi
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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Itagaki Shin-ichi
Department Of Biomedical Science Faculty Of Agriculyure The University Of Tokyo
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吉川 泰弘
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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