A Novel Enzyme-Linked Immunosorbent Assay for Quantification of Soybean β-Conglycinin, a Major Soybean Storage Protein, in Soybean and Soybean Food Products
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概要
- 論文の詳細を見る
Soybean (Glycine max L.) storage proteins are composed of two major components, β-conglycinin and glycinin, corresponding to 7S and 11S globulins, respectively. Recently, soybean β-conglycinin (7S globulin) has been reported to show beneficial functions in animals and human. To date, there is no method for the precise quantification of soybean β-conglycinin in processed food products or soybean seeds. We report here a novel method for this purpose. At first, antibodies specifically reactive to the subunits of β-conglycinin were prepared. And then, a direct enzyme-linked immunosorbent assay (ELISA) for the quantification of soybean β-conglycinin in processed foods and seeds was developed. In this assay, the sample was treated with sodium dodecyl sulfate sample buffer followed by dilution with phosphate-buffered saline. The diluted samples were poured and coated onto an ELISA plate and reacted with rabbit anti-β-conglycinin antibody and peroxidase-labeled anti-rabbit IgG. Finally, the bound peroxidase-labeled antibody was detected by colorimetric reaction. By using this system, it has been possible to measure soybean β-conglycinin concentrations in several processed food products. In addition, this simple quantification ELISA system was demonstrated to be adaptable for the quantification of β-conglycinin contents of various soybean cultivars.
- 日本ビタミン学会の論文
著者
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小川 智久
Graduate School Of Life Sciences Tohoku University
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Ogawa T
Animal Resource Science/veterinary Medical Science University Of Tokyo
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MARUYAMA Nobuyuki
Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
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Moriyama Tatsuya
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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OGAWA Tadashi
Graduate School of Agriculture, Kyoto University
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MAEBUCHI Motohiro
Graduate School of Agriculture, Kyoto University
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OZASA Sayaka
Graduate School of Agriculture, Kyoto University
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MACHIDORI Mika
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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OZASA Sayaka
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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MAEBUCHI Motohiro
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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URADE Reiko
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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TAKAHASHI Koji
Legume Breeding Laboratory, National Institute of Crop Science (NICS), National Agriculture and Bio-
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OGAWA Tadashi
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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Maebuchi Motohiro
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Maruyama Nobuyuki
Laboratory Of Food Biochemistry Faculty Of Fisheries Hokkaido University
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Urade R
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Ozasa Sayaka
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Machidori Mika
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Oda T
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Ogawa Tadashi
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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Takahashi Koji
Legume Breeding Laboratory National Institute Of Crop Science (nics) National Agriculture And Bio-or
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