Autodegradation of Protein Disulfide Isomerase
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概要
- 論文の詳細を見る
Protein disulfide isomerase (PDI) and its degradation products were found in HepG2,COS-1,and CHO-K1 cells. Whether or not the products were formed through autodegradation of PDI was examined, since PDI contains the CGHC motif, which is the active center of proteolytic activity in ER-60 protease. Commercial bovine PDI was autodegraded to produce a trimmed PDI. In addition, human recombinant PDI also had autodegradation activity. Mutant recombinant PDIs with CGHC motifs of which cysteine residues were replaced with serine or alanine residues were prepared. However, they were not autodegraded, suggesting the cysteine residues of motifs are necessary for autodegradation.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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KITO Makoto
Research Institute for Food Science, Kyoto University
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Moriyama Tatsuya
Research Institute For Food Science Kyoto University
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URADE Reiko
Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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Urade R
Laboratory Of Molecular Function Of Food Graduate School Of Agriculture Kyoto University
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OKUDO Hirokazu
Graduate School of Agriculture, Kyoto University
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URADE Reiko
Research Institute for Food Science, Kyoto University
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YASUNISHI Ayako
Research Institute for Food Science, Kyoto University
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OKUDO Hirokazu
Research Institute for Food Science, Kyoto University
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Kito M
Food Science Research Institute Fuji Oil Co. Ltd.
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Kito Makoto
Research Institute For Food Science Kyoto University
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Okudo Hirokazu
Graduate School Of Agriculture Kyoto University
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Yasunishi Ayako
Research Institute For Food Science Kyoto University
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Moriyama T
Kyoto Univ. Kyoto Jpn
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