Inhibition of Release of Arachidonic Acid by Monodansylcadaverine during Activation of Human Platelets by Collagen
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概要
- 論文の詳細を見る
Monodansylcadaverine (250μM), an inhibitor of transglutaminase, completely inhibited aggregation of human platelets induced by 2 μg/ml of collagen. Platelet responses necessary for aggregation (shape change, serotonin release, and phosphorylation of the 47, 000-Da protein) were also inhibited by this reagent. However, incorporation of [3H]putrescine into platelet proteins was very low and independent of the stimulation by collagen. It seems unlikely, therefore, that the transglutaminase reaction participates in platelet aggregation. Monodansylcadaverine inhibited the formation of thromboxane A2 with a similar median inhibitory concentration (about 120μM) for aggregation. When platelets prelabeled with [14C]arachidonic acid were stimulated by collagen, the radioactivities in thromboxane B2, hydroxyheptadecatrienoic acid, and hydroxyeicosatetraenoic acid increased. The increase was completely suppressed by the addition of monodansylcadaverine. Radioactivity in free arachidonic acid was very low throughout the experiments. From these results, it was concluded that monodansylcadaverine inhibited platelet aggregation by suppressing the release of arachidonic acid from phospholipids during platelet activation by collagen.
- 社団法人 日本農芸化学会の論文
著者
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KITO Makoto
Research Institute for Food Science, Kyoto University
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Kito Makoto
Research Institute For Food Science Kyoto University
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Matsuura Takao
Kyoto Red Cross Blood Center
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NARITA Hiroshi
Research Institute for Food Science, Kyoto University
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PARK Hwa
Research Institute for Food Science, Kyoto University
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TANAKA Ken-ichi
Kyoto Red Cross Blood Center
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