Preparation of Yeast Strains Displaying IgG Binding Domain ZZ and Enhanced Green Fluorescend Protein for Novel Antigen Detection Systems

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概要

• 論文の詳細を見る

• To develop novel immunofluorescence-labeling and antigen-detection systems, the ZZ domain derived from Staphylococcus aureus, which binds to the Fc part of immunoglobulin G, and enhanced green fluorescent protein (EGFP) were displayed on the cell surface of Saccharomyces cerevisiae by cell-surface engineering using the C-terminus 318 amino acids of Flo1 protein. Two systems were constructed, one for co-display of ZZ and EGFP, and one for display of a fusion protein of the two. In both cases, two proteins on the cell surface successfully retain their activities.

• 2003-11-25

著者

• Kondo Akihiko

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• Fukuda H

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Fukuda Hideki

  Organization Of Advanced Science And Technology Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Fukuda H

  Kobe University

• Kondo Akihiko

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda H

  Kobe Univ. Kobe Jpn

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Kondo Akihiko

  Department Of Bioactive Molecules National Institute Of Infectious Diseases

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• SHIMOJYO RYO

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• FURUKAWA HIROTAKA

  Chisso Corporation Yokohama Research Center

• Shimojyo Ryo

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Kondo Akihiko

  Department Of Applied Chemistry Kyushu Institute Of Technology

• Kondo Akihiko

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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