Thermal Stability and Starch Degradation Profile of α-Amylase from Streptomyces avermitilis

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概要

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• Amylases from Streptomyces are useful in the production of maltooligosaccharides, but they have weak thermal stability at temperatures higher than 40 °C. In this study, α-amylase (SAV5981 gene of Streptomyces avermitilis) was expressed from Streptomyces lividans 1326 and purified by ammonium sulfate fractionation followed by anionic chromatography (Q-HP sepharose). The properties of the purified SAV5981 amylase were determined by the starch-iodine method. The effect of metal ions on amylase activity was investigated. The optimal temperature shifted from 25 to 50 °C with the addition of the Ca2+ ion. The thermal stability of SAV5981 was also dramatically enhanced by the addition of 10 mM CaCl2. Improvement of the thermal stability of SAV5981 was examined by CD spectra in the presence and the absence of the Ca2+ ion. Thin-layer chromatography (TLC) analysis and HPLC analysis of starch degradation revealed that SAV5981 mainly produced maltose and maltotriose, not glucose. The maltoorigosaccharide-producing amylase examined in this study has the potential in the industrial application of oligosaccharide production.

• 公益社団法人 日本農芸化学会の論文

著者

• Kondo Akihiko

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• Nakashima Kazunori

  Organization of Advanced Science and Technology, Kobe University

• Kondo Akihiko

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Ogino Chiaki

  Department Of Chemical Science And Engineering Faculty Of Engineering Graduate School Of Engineering

• HWANG Sang

  Organization of Advanced Science and Technology, Kobe University

• OKAI Naoko

  Organization of Advanced Science and Technology, Kobe University

• OKAZAKI Fumiyoshi

  Organization of Advanced Science and Technology, Kobe University

• MIYAKE Michiru

  Organization of Advanced Science and Technology, Kobe University

• HARAZONO Koichi

  R&D Center, Nagase & Co., Ltd.

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