Preparation of Immobilized Papain Using Thermosensitive Latex Particles
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概要
- 論文の詳細を見る
Poly(methul methacrylate/N-isopropylacrylamide/methacrylic acid) [P(MMA/NIPAM/MAA)] and poly(styrene/N-isopropylacrylamide/methacrylic acid)[P(St/NIPAM/MAA)] latex particles were prepared by emulsifier-free emulsion polymerization. These submicrometer-size latex particles showed a reversible transition between dispersion and flocculation (thermoflocculation) by lowering and raising temperature. This thermosensitivity originated from the thermosensitive nature of NIPAM. Papain was covalently immobilized onto the thermosensitive latex particles with high efficiency by the carbodimide method. Moreover, the latex particles with covalently immobilized papain were separated by thermoflocculation, dispersed by lowering temperature and reused. Papain immobilized on the P(MMA/NIPAM/MAA) latex particles showed an extremely high activity toward casein and retained the activity without any definite loss during repeated thermocycles.
- 公益社団法人日本生物工学会の論文
- 1994-09-25
著者
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Kondo Akihiko
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Higashitani Ko
Department Of Applied Chemistry Kyushu Institute Of Technology:(present Address)department Of Chemic
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IMURA KOUICHI
Department of Applied Chemistry, Kyushu Institute of Technology
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NAKAMA KOUZO
Department of Applied Chemistry, Kyushu Institute of Technology
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Kondo Akihiko
Department Of Applied Chemistry Kyushu Institute Of Technology
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Higashitani Ko
Department Of Applied Chemistry Kyushu Institute Of Technology
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Nakama Kouzo
Department Of Applied Chemistry Kyushu Institute Of Technology
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Imura Kouichi
Department Of Applied Chemistry Kyushu Institute Of Technology
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Kondo Akihiko
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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