Induction of apoptosis associated with chromosomal DNA fragmentation and caspase-3 activation in leukemia L1210 cells by TiO_2 nanoparticles(MISCELLANEOUS)
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概要
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We investigated the effects of nanosized TiO_2 particles on the death of mouse leukemia L1210 cells. TiO_2 particles suppressed proliferation and induced cell death, as measured by lactate dehydrogenase (LDH) release into the culture medium. Chromatin condensation, which is typical of the initiation of cell death, was observed in approximately 14% cells cultured with titanium dioxide (TiO_2) particles for 12 h. Furthermore, giant DNA fragments of approximately 2 Mbp and high-molecular-weight DNA fragments between 100 kbp and 1 Mbp were observed in cells cultured for 18 h with TiO_2 particles. These giant and high-molecular-weight DNA fragments were further degraded into smaller DNA fragments, appearing as DNA ladders. Corresponding to the generation of DNA fragments, caspase-3 activity increased in cells treated with TiO_2 particles. TiO_2 particle-induced LDH release was not inhibited by cytochalasin D, an inhibitor of endocytosis. These results suggest that nanosized TiO_2 particles can induce apoptosis associated with DNA fragmentation and caspase-3 activation and that TiO_2 particle-induced apoptosis is not caused by endocytosis but is associated with contact of the particles with the cell surface.
- 公益社団法人日本生物工学会の論文
著者
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Hashii Minako
Department Of Biophysical Genetics Kanazawa University Graduate School Of Medicine
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Higuchi Yoshihiro
Department Of Applied Quantum Physics Faculty Engineering Graduate School Of Kyusyu University
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Ogino Chiaki
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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Takaki Keiko
Division Of Life Sciences Graduate School Of Science And Technology Kanazawa University
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Ogino Chiaki
Department Of Chemical Science And Engineering Faculty Of Engineering Graduate School Of Engineering
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HASHII Minako
Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine
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Shimizu Nobuaki
Division of Biological Measurement and Applications, Institute of Nature and Environmental Technology, Kanazawa University
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Takaki Keiko
Division of Biological Measurement and Applications, Institute of Nature and Environmental Technology, Kanazawa University
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