S28. Targeted sonodynamic therapy using protein-modified TiO_2 nanoparticles(Oral Presentation)
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概要
- 論文の詳細を見る
Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO_2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO_2 NPs activated by external ultrasound irradiation (called TiO_2/US treatment). The present study first examined the uptake behavior of TiO_2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. Next, the effect of the TiO_2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm^2, 30 s) to the cells which incorporated the TiO_2 NPs. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO_2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO_2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm^2 for 60 s. As a result of the treatment repeated 5 times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions.
- 日本ソノケミストリー学会の論文
- 2011-10-15
著者
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Ogino Chiaki
Department Of Chemical Science And Engineering Faculty Of Engineering Graduate School Of Engineering
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Shimizu Nobuaki
Institute of Natuare and Environmental Technology, Kanazawa University
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Ninomiya Kazuaki
Institute of Natuare and Environmental Technology, Kanazawa University
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Oshima Shuhei
Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University
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Sonoke Shiro
Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University
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Kuroda Shun-ichi
Department of Bioengineering Sciences, Graduate School of Bioagricultural Sciences, Nagoya University
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