Purification and Characterization of an Antibiotic Substance Produced from Rhizopus oligosporus IFO 8631
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概要
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We obtained a purified antibiotic protein from the submerged cultivation broth of Rhizopus oligosporus IFO 8631 by using CM-Cellulofine chromatography and HPLC.The antibiotic did not show a broad spectrum of activity, but it was very active against some of the Bacillus species, especially against Bacillus subtillis (B.natto) at a very low concentration (less than 1 ppm). It also showed ctivity against other gram-positive bacteria, including Staphylococcus aureus and Streptococcus cremoris. The purified antibiotic was a simple protein of about 5,500 in molecular weight, the amino acid component being characteristically high in cystine content. This high cystine content contributed to the stability of the antibiotic over a wide pH range and against strong heating (50% of the activity remained after boiling for 1 hr).
- 社団法人日本農芸化学会の論文
- 1992-01-23
著者
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KOSEKI Takuya
National Research Institute of Brewing
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OKAZAKI NAOTO
National Research Institute of Brewing
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KOBAYASI Sin-ya
National Research Institute of Brewing
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