Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain
スポンサーリンク
概要
- 論文の詳細を見る
The recent finding that [1-Me-His^2] TRH is present in the carp brain prompted us to ascertain whether methylated TRH also exists in the frog brain. Samples were prepared from whole brain tissues of prometamorphic and climax tadpoles, and juvenile and adult frogs by acid extraction. Each sample was subjected to reverse-phase high-performance liquid chromatogrphy. The eluates were assayed for TRH using two kinds of antibodies against TRH, one showing 100% cross-reactivity with both methylated TRHs and the other showing 3% and 43% cross-reactivities with [1-Me-His^2] TRH and [3-Me-His^2] TRH, respectively. Both the distribution and amount of immunoreactive TRH in the eluates of each sample did not vary between the assays using the two different antibodies. In both cases, a single peak with a similar immunoreactivity appeared at a position similar to that of synthetic TRH. Thus, it is concluded that brains of larval and adult bullfrog contain no appreciable amount of methylated TRH.
- 社団法人日本動物学会の論文
- 1993-08-16
著者
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KIKUYAMA Sakae
Department of Biology, School of Education, Waseda University
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Kikuyama Sakae
Department Of Biology Faculty Of Science Shizuoka University
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TANAKA Shigeyasu
Department of Biology, Faculty of Science, Shizuoka University
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Yanagisawa Tadashi
Department of Mechanical Engineering, Shizuoka University
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Tanaka Shigeyasu
Department Of Biology Faculty Of Science Shizuoka University
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Tanaka S
Univ. Occupational And Environmental Health Kitakyushuu Jpn
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Yanagisawa Tadashi
Department Of Agricultural Chemistry Faculty Of Agriculture Utsunomiya University
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Nakajima Keisuke
Department Of Information Science Faculty Of Engineering Utsunomiya University
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Nakajima Keisuke
Department Of Biology School Of Education Waseda University
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Yanagisawa T
Department Of Agricultural Chemistry Utsunomiya University
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TANAKA Shigeyasu
Department of Anatomy, Josai Dental College
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