Regional Concentration and Chromatographic Characterization of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in the Brain of the Bullfrog, Rana catesbeiana(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a regulatory neuropeptide which functions as a hypothalamic factor for pituitary hormone release, and as a neurotransmitter, neuromodulator and neurotrophic factor in both frogs and mammals. This study examined the quantitative distribution and chromatographic characterization of immunoreactive PACAP in the central nervous system (CNS) of the bullfrog, Rana catesbeiana, using an enzyme immunoassay (EIA), named avidin-biotin complex detectable EIA for PACAP, and high-performance liquid chromatographic (HPLC) analysis. The brain of adult bullfrogs contained relatively high levels of immunoreactive PACAP (344.63 pmol/g wet weight of tissue). The average concentrations of immunoreactive PACAP in the regions of the telencephalon, diencephalon, tectum, cerebellum, rhombencephalon, and spinal cord were 213.84, 767.14, 524.94, 192.71, 237.67, and 362.04 pmol/g wet weight of tissue, respectively. The concentrations of immunoreactive PACAP increased with the brain development during metamorphosis, and the concentration of immunoreactive PACAP in the brain of tadpoles at the end of metamorphosis was approximately 200 pmol/g wet weight of tissue. The predominant form of immunoreactive PACAP in the CNS of adult and tadpole was eluted closely with synthetic PACAP38, but another smaller immunoreactivity also appeared in a the fraction, which corresponded to the retention time of synthetic PACAP27, as analyzed by reverse-phase HPLC.
- 社団法人日本動物学会の論文
- 2003-08-25
著者
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KIKUYAMA Sakae
Department of Biology, School of Education, Waseda University
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Kikuyama Sakae
Department Of Biology Faculty Of Science Shizuoka University
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Onoue S
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Toho University
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Onoue Satomi
Itoham Foods Inc. Central Research Institute
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Matsuda K
Laboratory Of Regulatory Biology Graduate School Of Science And Engineering University Of Toyama
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MATSUDA KOUHEI
Department of Brology, Faculty of Science, Toyama University
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Onoue Satomi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Toho University
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MOCHIZUKI TOHRU
Shizuoka College of Pharmacy
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UCHIYAMA MINORU
Department of Oral Physiology
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Matsuda Kouhei
Department Of Biology Faculty Of Science Toyama Univercity
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Uchiyama M
Laboratory Of Regulatory Biology Graduate School Of Science And Engineering University Of Toyama
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Uchiyama Minoru
Department Of Oral Physiology Nippon Dental University
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Uchiyama Minoru
Department Of Biology Faculty Of Science Toyama Univercity
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Kawaura Hiromi
Department of Biology, Faculty of Science, Toyama University
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Kashimoto Kazuhisa
ItoHam Foods Inc., Central Research Institute
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Onoue S.
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Toho University
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Kawaura Hitomi
Department Of Biology Faculty Of Science Toyama University
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Mochizuki Tohru
Shizuoka Cancer Center Hospital And Research Institute
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Kashimoto Kazuhisa
Itoham Foods Inc. Central Research Institute
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