Apoptosis in neural tube during normal development of medaka
スポンサーリンク
概要
- 論文の詳細を見る
Neural apoptosis in normal medaka (Oryzias latipes) embryos at stage 20 (the neural rod step in brain development), stage 27 (the neural tube step in brain development), and stage 29 (the late embryonic brain step in brain development) were examined, and the results were compared with those reported for other vertebrates, particularly zebrafish. Apoptotic cells were identified using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling), by which fragmented DNA, which is characteristic of apoptotic cells, is labeled. Whole-mount TUNEL showed the following gross pattern of apoptotic changes during development. At stage 20, numerous single apoptotic cells were distributed diffusely in the entire regions of the brain. At stage 27, in addition to the diffusely distributed apoptotic cells, several large aggregates of TUNEL-positive structures (apoptotic centers) appeared in the brain and eyes. Histological observations of sections of the stained embryos revealed that apoptotic centers are present in the zona limitans intrathalamica, the ventral region of the optic stalk, several sites of the floor plate, and the retina. At stage 29, both single apoptotic cells and apoptotic centers decreased in number. This apoptotic pattern in medaka embryos is generally similar to that in zebrafish embryos, but apoptosis in the nervous system continues for a longer period in medaka than in zebrafish. Apoptosis in the vertebrate nervous system seems to be ubiquitous, random and probabilistic in nature.
- 名古屋大学の論文
著者
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Maeda Keiko
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Ishikawa Yuji
National Inst. Radiological Sci. Chiba Jpn
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Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Yasuda Takako
Res. Center For Charged Particle Therapy National Inst. Of Radiological Sciences
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Yasuda Takako
National Institute Of Radiological Sciences
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Matsumoto Atsuko
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Matsumoto Atsuko
National Food Research Institute
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Ishikawa Yuji
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Maeda Keiko
National Institute of Radiological Sciences
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Maruyama Kouichi
National Institute of Radiological Sciences
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Yasuda Takako
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Maruyama Kouichi
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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