Radiation-Induced Brain Cell Death Can Be Observed in Living Medaka Embryos
スポンサーリンク
概要
- 論文の詳細を見る
Medaka (Oryzias latipes) embryos at 25-26 and 28-30 stages were irradiated with a single acute dose of 10 Gy of X-ray, which is lower than the LD_<50> of the embryos. The effects on developing brains were examined under a stereomicroscope in living embryos until hatching. All the irradiated embryos survived; however, from 6 to 35h after X-ray irradiation, massive clusters of optically opaque and round cells were observed either in the entire brain region (when irradiated at 25-26 stages) or mainly in the optic tectum (when irradiated at 28-30 stages). Histological examination and TUNEL showed that these cells are clusters of dead cells. These dead cell clusters disappeared thereafter, and the irradiated embryos continued to develop apparently normally. The grown irradiated embryos, however, had smaller brains and eyes than the nonirradiated control embryos. At hatching, the irradiated embryos exhibited histological abnormalities in the brain, particularly in the torus longitudinalis, and in the retina, although most of them hatched normally and survived. The results indicate that brain cell death and a reduced brain size can be observed in living irradiated embryos, and suggest that the medaka embryo is useful for screening the developmental neurotoxicity effects of various hazardous factors.
- 日本放射線影響学会の論文
著者
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FUSHIKI SHINJI
Department of Clinical Pathology, Kyoto Prefectural University of Medicine
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Ishikawa Yuji
National Inst. Radiological Sci. Chiba Jpn
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Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Fushiki Shinji
Department Of Clinical Pathology Kyoto Prefectural University Of Medicine:department Of Pathology Ky
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Yasuda Takako
Res. Center For Charged Particle Therapy National Inst. Of Radiological Sciences
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Yasuda Takako
National Institute Of Radiological Sciences
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Matsumoto Atsuko
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Matsumoto Atsuko
National Food Research Institute
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Ishikawa Yuji
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Maruyama Kouichi
National Institute of Radiological Sciences
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Yasuda Takako
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Aoki Kazuko
National Institute Of Radiological Sciences
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Fushiki S
Department Of Pathology And Applied Neurobiology Graduate School Of Medical Science Kyoto Prefectual
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HYODO-TAGUCHI Yasuko
National Institute of Radiological Sciences
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Hyodo‐taguchi Y
National Institute Of Radiological Sciences
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Maruyama Kouichi
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
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