Matrix Cells Dissociated from the Mouse Forebrain Vesicle Reaggregate to Form Neural Tube-like Organoid Structures in vitro

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An in vitro study employing dissociated matrix cells obtained from the rostral part of mouse neural tube or forebrain vesicle was done in an attempt to elucidate their cellular characteristics. We found a reaggregate formation with an organoid architecture. The organization of the reaggregate was studied by transmission as well as scanning electron microscopy, and by immunocytochemistry. After three days in a stationary culture, dissociated matrix cells formed a spherical, organoid reaggregate having a central lumen. The size of the reaggregate varied from approximately 100μm to 200μm. The transmission electron microscopy revealed that the reaggregate wall is composed of two types of cells; one has an electron-lucent cytoplasm with few organelles except for polysomes, whereas the other contains an electron-dense cytoplasm with many microtubules and mitochondria. The latter type of cell was located in the marginal portion of the wall intermingled with a large number of cellular processes. These cells were immunostained with anti-neurofilament antibodies and regarded as neuroblasts. The luminal surface was composed predominantly of the former cell type showing well-developed junctional complexes in between them and having cilia on its luminal side. Mitotic figures were frequently demonstrable adjacent to the lumen.
日本組織細胞化学会の論文
1995-10-01