TWO-WAVE-LENGTH-SCANNING METHOD IN FEULGEN CYTOPHOTOMETRY
スポンサーリンク
概要
- 論文の詳細を見る
In Feulgen stained specimens, non-specific light loss is always present, which can be detected and measured quantitatively by scanning with a light spot at 450 nm. Subtraction of the total "absorbance" at 450 nm from that of 550 nm virtually eliminates the effects of non-specific light loss and gives more accurate value of DNA content of a Feulgen stained cell. This is called two-wave-length-scanning method. An automatic instrument to perform two-wave-length-scanning measurements is described. By this method measurements were carried out on DNA contents of Purkinje cells and inner granule neurons in cerebellum of man and rat at various stages of postnatal life, and the rule of strict constancy of DNA content is confirmed. Based on discussions on variotus sources of the non-specific light loss, the use of this method is recommended when high accuracy is required in measurements of Feulgen cytophotometry.
- 日本組織細胞化学会の論文
著者
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Yoshida Satoru
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fujita Setsuya
Department Of Hygiene Gifu University School Of Medicine
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Yoshida Satoru
Department Of Aerospace Engineering Nagoya University
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KITAMURA TADAHISA
Department of Pathology, Kyoto Prefectural University of Medicine
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Fukuda Masaru
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Kitamura Tadahisa
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fujita Setsuya
Department Of Dynamic Pathology Research Institute For Neurological Diseases And Geriatrics Kyoto Pr
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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FUKUDA MASARU
Department of Pathology, Kyoto Prefectural University of Medicine
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