DEOXYRIBONUCLEIC ACID SYNTHESIS IN REGENERATING RAT LIVER STUDIED BY CYTOFLUOROMETRY IN COMBINATION WITH TRITIATED THYMIDINE AUTORADIOGRAPHY

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Combination of DNA cytofluorometry and tritiated thymidine autoradiography enables us to measure absolute length of S-phase in a cell population, with a single sampling. Different ploidy classes in a mixed population can be measured separately according to difference in DNA contents. Proliferating cells in the regenerating rat liver are studied by this method and the S-phase is estimated at 6.7 hr and 8 hr for diploid and tetraploid classes, respectively. After cumulative labeling from 20 to 35 hr after hepatectomy, it was found that most labeled hepatocytes do not retturn to the diploid level, thereby indicating that the regenerative proliferation of the hepatocytes is not a reversible process. In the initial stage up to 30 hr after hepatectomy, both diploid and tetraploid cells synthesize DNA. From 30 hr to 35 hr after hepatectomy, DNAsynthetic cells are mostly of tetraploid class. At 48 post-operative hours, again both diploid and tetraploid cells are synthesizing DNA. Throughout the regenerative period, the tetraploid cells synthesize DNA, divide and return to the tetraploid G-1 cells. They seem to play the most important role in the regeneration of the adult rat liver.
日本組織細胞化学会の論文