COMBINED DNA AND PROTEIN CYTOFLUOROMETRY OF GYNECOLOGICAL SMEARS : QUANTITATIVE IDENTIFICATION OF TUMOR CELLS AND DISCRIMINATION OF INTRAEPITHELIAL NEOPLASIA FROM INVASIVE CANCER
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概要
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Both DNA and protein levels of cells on the cytological specimens obtained from 12 normal individuals, 3 cases of cervical dysplasia, 2 cases of carcinoma in situ, 11 cases of cervical cancer and 4 cases of endometrial carcinoma were measured by combined DNA and protein cytofluorometry. In cervical dysplasia, the percentage of cells in which DNA values exceeded normal diploid-tetraploid region was high in accordance with the intensity of epithelial atypia. The DNA and protein distribution patterns of severe dysplasia were mostly similar to those of carcinoma in situ. In all the cases of uterine cancer measured, both DNA and protein levels of representative cells were abnormally elevated, exhibiting hyperploidy and aneuploidy in their DNA and protein content. Moreover, a significant positive correlation was observed between DNA and protein values in a linear regression analysis. The parallelism between DNA and protein values was more evident in invasive cervical cancer than in carcinoma in situ. Therefore, it is suggested that an abnormal elevation of both DNA and protein levels in neoplastic cells may berelated to tumor invasiveness. The present study suggests that combined DNA and protein cytofluorometry enables us to identify uterine neoplastic cells quantitatively and facilitates discriminate ion of intraepithelial neoplasia from invasive cancer with respect to their DNA and protein levels.
- 日本組織細胞化学会の論文
著者
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KATO YOSHIKO
Department of Bacteriology, Osaka City University Medical School
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FUKUDA Masaru
Department of Pathology, Fukui Medical University
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Kato Yoshiko
Department Of Obstetrics And Gynecology Kyoto Prefectural University Of Medicine
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KIMURA Junji
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
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OKADA Hiroji
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
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HARADA KIYOYUKI
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
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NAKANISHI KAZUO
Department of Pathology, Kyoto Prefectural University of Medicine
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FUJITA SETSUYA
Department of Pathology, Kyoto Prefectural University of Medicine
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Fujita Setsuya
Department Of Hygiene Gifu University School Of Medicine
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Fujita Setsuya
Dept. Of Ophthalmol. And Pathol. Kyoto Pref. Univ. Med.
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Fujita Setsuya
Louis Pasteur Center For Medical Research
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Kimura J
Department Of Obstetrics And Gynecology Kyoto Prefectural University Of Medicine
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Kimura Junji
Department Of Chemistry And Biological Science College Of Science And Engineering Aoyama Gakuin Univ
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Okada H
Kyoto Prefectural Univ. Medicine Kyoto Jpn
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Okada Hiroji
Department Of Obstetrics And Gynecology Kyoto Prefectural University Of Medicine
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Fujita S
Louis Pasteur Center For Medical Research
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Fukuda Masaru
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Nakanishi K
Food Research And Development Laboratories Ajinomoto Co. Inc.
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Nakanishi Kazuo
Department Of Orthopaedic Surgery Okayama University Hospital Of Medicine And Dentistry
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Nakanishi Kazuo
Dept Of Pathology Kyoto Pref. Univ. Of Med.
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Harada Kiyoyuki
Department Of Obstetrics And Gynecology Kyoto Prefectural University Of Medicine
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Fujita Setsuya
Department Of Dynamic Pathology Research Institute For Neurological Diseases And Geriatrics Kyoto Pr
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Nakanishi Kazuo
Department Of Anesthesiology And Resuscitology Ehime University Graduate School Of Medicine
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Okada Hiroji
Dept. Of Obstet & Gynecol. Kyoto Prefectural Univ. Med.
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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KATO Yoshiko
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine
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FUKUDA MASARU
Department of Pathology, Kyoto Prefectural University of Medicine
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- COMBINED DNA AND PROTEIN CYTOFLUOROMETRY OF GYNECOLOGICAL SMEARS : QUANTITATIVE IDENTIFICATION OF TUMOR CELLS AND DISCRIMINATION OF INTRAEPITHELIAL NEOPLASIA FROM INVASIVE CANCER