Demonstration of Biological Aggressiveness of Bone Giant Cell Tumor by the Comparative Study of Immunohistochemieal Detection of DNA-instability and Cortical Bone Destruction by CT
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概要
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The degree of DNA-instability as revealed by the immunohistochemical staining with anti-single-stranded DNA antibody after acid hydrolysis (DNA-instability test) was used as a marker of malignancy. This was applied to benign (4 osteochondroma and 4 enchondroma cases), border-line (23 bone giant cell tumor, BGCT cases), and malignant (6 chondrosarcoma and 6 osteosarcoma cases) neoplastic lesions. The expression of tumor oncogene, c-myc was detected immunohistochemically. Proliferative activity was evaluated by PCNA-immunohistochemistry, and the quantitative analysis of the number, mean area, mean total area, the largest area, and maximum shape-irregularity of AgN0Rsin a rtucleus were performed for all these cases. The results for 19 BGCT 82.6% cases, 6 chondrosarcoma cases (100%), and 6 osteosarcoma cases (100%) were positive with the DNA-instability test, indicating their malignancy. All benign tumor cases were negative with the DNA-instability test. Reflecting the malignant character, all chondrosarcoma cases, all osteosarcoma cases and the BGCT cases positive with the DNA-instability test showed statistically higher values of PCNA-index; and all AgNORs- parameters in comparison to those for benign tumor cases, and c-myc was positive for 66.7%, and 26.3%, of them, respectively. But these values for BGCT with positive and negative DNA-instability test results showed no statistically meaningful differences. All the BGCT cases negative with DNA-instability test were negative for c-myc expression. Among the BGCT cases positive with DNA-instability test, 18 cases (94.7%) showed cortical bone destruction by computed tomography (CT), and 5 cases (26.3%) showed extra-osseous expansion. No such radiographic changes were detected among the BGCT cases negative With DNA-instability test. Among 18 BGCT cases with cortical bone destruction, 5 cases (27.8%) showed tumor recurrences, and 2 cases (11.1%) showed lung metastases. These results indicate that the majority of BGCT cases are malignant and the detection of cortical bone destruction by CT is a sensitive clinical marker to detect them.
- 日本組織細胞化学会の論文
著者
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FUKUDA Masaru
Department of Pathology, Fukui Medical University
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BABA Hisatoshi
Department of Orthopaedic Surgery, University of Fukui
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Noriki Sakon
Division Of Tumor Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Universi
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Noriki Sakon
Department Of Pathology Fukui Medical University
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Baba Hisatoshi
Department Of Orthopedics And Rehabilitation Medicine Fukui University School Of Medicine
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Baba Hisatoshi
Department Of Orthopaedic Surgery Fukui University
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Baba Hisatoshi
Department Of Orthopaedic Surgery Fukui Medical School
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Baba Hisatoshi
Department Of Orthopedic Surgery Fukui Medical University
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Fukuda M
Glycobiology Program Cancer Research Center The Burnham Institute
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IMAMURA Yoshiaki
Department of Pathology, University of Fukui
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Noriki Sakon
First Department Of Pathology Fukui Medical University
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IMURA SHINICHI
Department of Orthopaedic Surgery, Fukui Medical School
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Imamura Yoshiaki
Division Of Surgical Pathology Faculty Of Medical Sciences University Of Fukui
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Imamura Yoshiaki
Department Of Clinical Pathology University Of Fukui
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Azuchi Miki
Department of Orthopaedic Surgery, School of Medicine, Fukui Medical University
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Imamura Y
Tumour Pathology Fukui University Faculty Of Medical Sciences
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Fukuda M
Department Of Surgical Pathology University Hospital Faculty Of Medicine University Of Fukui
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Imura Shinichi
Department Of Orthopaedic Surgery School Of Medicine Fukui Medical University
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Imura Shinichi
Department Of Orthopaedic Surgery Fukui Medical School
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Fukuda Minoru
Department Of Anatomy Kyorin University School Of Medicine
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Azuchi Miki
Department Of Orthopaedic Surgery School Of Medicine Fukui Medical University
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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