CYTOFLUOROMETRIC QUANTITATION OF THE HEMOGLOBIN AND DNA CONTENTS OF A SINGLE ERYTHROID CELL
スポンサーリンク
概要
- 論文の詳細を見る
Standardization and improvements upon a previously reported method (Fukuda et al. 1975) for the cytofluorometric quantitation of the hemoglobin (Hb) and nuclear DNA contents of a single erythroid cell were described. Intracellular Hb was converted to fluorescent porphyrin by irradiation with violet light (λ 405 nm, fluence rate, 12×10^<-2> mJ/sec/mm^2) in the presence of SH-donor (mercaptoethylamine hydrochloride, MEA). The intensity of porphyrin fluorescence increased with prolonged photochemical reaction reaching a plateau level at 40 min (total 288 mJ/mm^2). With this improved method, maximum fluorescence for erythroid cells was found to be about 15 times stronger in intensity than with the previously reported method. Under maximum conditions a linear relationship was found between fluorescence intensity and Hb content (MCH) through the use of the peripheral erythrocytes of different adult male animals (sheep, man, and chick). The fluorescences of porphyrin and Feulgen DNA were measured without interference with each other, through analysis of the emission spectra on a erythroblast lightly stained with pararosaniline Feulgen after the optimum photochemical reaction.
- 日本組織細胞化学会の論文
著者
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Isemura Takuji
Department Of Pathology Kyoto Prefectural University Of Medicine
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FUKUDA Masaru
Department of Pathology, Fukui Medical University
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NAKANISHI KAZUO
Department of Pathology, Kyoto Prefectural University of Medicine
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FUJITA SETSUYA
Department of Pathology, Kyoto Prefectural University of Medicine
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Fujita Setsuya
Department Of Hygiene Gifu University School Of Medicine
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Fujita Setsuya
Dept. Of Ophthalmol. And Pathol. Kyoto Pref. Univ. Med.
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Fujita Setsuya
Louis Pasteur Center For Medical Research
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Fujita S
Louis Pasteur Center For Medical Research
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MARUO NAOYUKI
Department of Pathology, Kyoto Prefectural University of Medicine
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BOHM NORBERT
Department of Pathology, Kyoto Prefectural University of Medicine
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Maruo Naoyuki
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fukuda Masaru
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Bohm Norbert
Department Of Pathology Kyoto Prefectural University Of Medicine:the Institute Of Pathology Universi
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Nakanishi K
Food Research And Development Laboratories Ajinomoto Co. Inc.
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Nakanishi Kazuo
Department Of Orthopaedic Surgery Okayama University Hospital Of Medicine And Dentistry
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Nakanishi Kazuo
Dept Of Pathology Kyoto Pref. Univ. Of Med.
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Fujita Setsuya
Department Of Dynamic Pathology Research Institute For Neurological Diseases And Geriatrics Kyoto Pr
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Nakanishi Kazuo
Department Of Anesthesiology And Resuscitology Ehime University Graduate School Of Medicine
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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FUKUDA MASARU
Department of Pathology, Kyoto Prefectural University of Medicine
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