Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy
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概要
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The aim of the present study was to investigate the quantity and quality of hydroxyapatite (HA) in the zone of calcified cartilage of articular cartilage and underlying subchondral bone by Fourier transform infrared microscopy (FTIRM). We hypothesized that the amount of HA deposited in osteoarthritis (OA) might differ from normal cartilage, and that such differences might be significant in the pathology of OA. The tibial epiphysis of the male Hartley strain for the spontaneous OA model and the control Strain 13 guinea pigs (12-month old) were examined. The mineral : matrix ratio was determined by integrating areas of υ1, υ3 bands at 900-1200 cm^<-1> to Amid 1 band at 1585-1725 cm^<-1>. The CO_3 : PO_4 ratio was determined by integrating areas of υ2 carbonate band at 850-900 cm^<-1> to υ1, υ3 bands. In Hartley strain, the mean mineral : matrix ratio of the medial side was significantly lower than the lateral side at the zone of the calcified cartilage and subchondral bone. The mean CO_3 : PO_4 ratio was significantly higher in the medial side than the lateral one at the zone of the calcified cartilage and subchondral bone. In contrast, the results obtained from Strain 13 showed no significant differences between the two sides. Our results showed diminished deposition and increased maturation of HA in Hartley strain. These findings may correlate with the pathological process of knee OA, and indicate that FTIRM is a useful tool for determining the variability of HA in OA.
- 日本組織細胞化学会の論文
著者
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MIYOSHI Norio
Department of Pathology, Fukui Medical University
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FUKUDA Masaru
Department of Pathology, Fukui Medical University
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BABA Hisatoshi
Department of Orthopaedic Surgery, University of Fukui
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IMAMURA Yoshiaki
Division of Surgical Pathology, Faculty of Medical Sciences, University of Fukui
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KOBAYASHI Shigeru
Department of Human Geography, Graduate School of Letters, Osaka University
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Noriki Sakon
Division Of Tumor Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Universi
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Noriki Sakon
Department Of Pathology Fukui Medical University
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Sato Mitsuhiko
Departments Of Orthopaedics And Rehabilitation Medicine University Of Fukui:departments Of Pathology
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Baba Hisatoshi
Department Of Orthopedics And Rehabilitation Medicine Fukui University School Of Medicine
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Baba Hisatoshi
Department Of Orthopaedic Surgery Fukui University
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Baba Hisatoshi
Department Of Orthopaedic Surgery Fukui Medical School
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Baba Hisatoshi
Department Of Orthopedic Surgery Fukui Medical University
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Fukuda M
Glycobiology Program Cancer Research Center The Burnham Institute
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IMAMURA Yoshiaki
Department of Pathology, University of Fukui
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UCHIDA Kenzo
Department of Urology, Tokyo Metropolitan Government Officials Mutual Aid Association Aoyama Hospita
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Noriki Sakon
First Department Of Pathology Fukui Medical University
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YAYAMA TAKAFUMI
Department of Orthopaedics and Rehabilitation Medicine, Fukui University Faculty of Medical Sciences
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NEGORO KOHEI
Department of Orthopaedics and Rehabilitation Medicine, Fukui University Faculty of Medical Sciences
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FUJIMOTO Michiyo
Department of Orthopaedic Surgery, Fukui University
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Wada Makoto
Departments of Orthopaedics and Rehabilitation Medicine University of Fukui
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Imamura Yoshiaki
Division Of Surgical Pathology Faculty Of Medical Sciences University Of Fukui
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Imamura Yoshiaki
Department Of Clinical Pathology University Of Fukui
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Kobayashi Shigeru
Department Of Orthopedics And Rehabilitation Medicine Fukui University School Of Medicine
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Imamura Y
Tumour Pathology Fukui University Faculty Of Medical Sciences
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Fukuda M
Department Of Surgical Pathology University Hospital Faculty Of Medicine University Of Fukui
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Wada Makoto
Department Of Chemistry Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Uchida Kenzo
Department Of Orthopedics And Rehabilitation Medicine Fukui University School Of Medicine
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Uchida Kenzo
Department Of Endocrinology Kanazawa Medical University
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Negoro Kohei
Department Of Orthopaedics And Rehabilitation Medicine Fukui University Faculty Of Medical Sciences
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Miyoshi N
Department Of Surgical Pathology University Hospital Faculty Of Medicine University Of Fukui
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Miyoshi Norio
Department Of Chemistry Okayama University Of Science
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Yayama Takafumi
Department Of Orthopedics And Rehabilitation Medicine Fukui University School Of Medicine
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Fukuda Minoru
Department Of Anatomy Kyorin University School Of Medicine
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Wada Makoto
Osaka Univ. Suita‐shi Jpn
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Fujimoto Michiyo
Departments Of Orthopaedics And Rehabilitation Medicine University Of Fukui
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Miyosi Norio
Department Of Pathology Fukui Medical School
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Yayama Takafumi
Department Of Orthopaedics And Rehabilitation Medicine The University Of Fukui
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Sato Mitsuhiko
Department Of Biology Faculty Of Science Tokyo Metropolitan University
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Miyoshi Norikazu
Department Of Chemistry Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Kobayashi Shigeru
Deparment Of Oral Anatomy Kyushu Dental College
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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Yayama Takafumi
Department Of Orthopaedics And Rehabilitation Medicine Faculty Of Medical Sciences University Of Fukui
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