COMBINATION OF FEULGEN CYTOFLUOROMETRY WITH ^3H-THYMIDINE AUTORADIOGRAPHY
スポンサーリンク
概要
- 論文の詳細を見る
A new method is described to measure DNA contents of labeled and unlabeled cells in autoradiographs without removing silver grains. Smears of regenerating rat liver, flash-labeled with ^3H-thymidine, were made on cover glasses, stained by Feulgen method and processed for autroadiography. The cover glass was mounted on a slide glass, with the emulsion side facing downward, so that Feulgen cytofluorometry with incident light excitation can be carried out without interference by silver grains in the emulsion. Transmitted light illumination which can be turned on at any time allowed observation of cell image and silver grains under the same cell. Cytofluorometric data obtained by this method proved that this is a simple, rapid and reliable method of determining DNA contents on labeled and unlabeled cells in autoradiographs. Possibility is discussed to apply this method generally for determining amount of fluorescent substances in combination with autoradiography.
- 日本組織細胞化学会の論文
著者
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Yoshida Satoru
Department Of Surgery Yokohama City University Hospital And School Of Medicine
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FUKUDA Masaru
Department of Pathology, Fukui Medical University
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HATTORI TAKANORI
Department of Pathology, Shiga University of Medical Science
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ASHIHARA TSUKASA
Department of Pathology, Kyoto Prefectural University of Medicine
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Yoshida Satoru
Department Of Pathology Kyoto Prefectural University Of Medicine
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FUJITA SETSUYA
Department of Pathology, Kyoto Prefectural University of Medicine
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Fujita Setsuya
Department Of Hygiene Gifu University School Of Medicine
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Hattori Takanori
Department Of Pathology Fukui Medical School
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Ashihara Tsukasa
Department Of Pathology Kyoto Prefectural University Of Medicine
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Yoshida Satoru
Department Of Aerospace Engineering Nagoya University
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Fukuda Masaru
Department Of Pathology Kyoto Prefectural University Of Medicine
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Fukuda Masaru
Department Of Pathology Fukui Medical School
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Hattori Takanori
Department Of Pathology Kyoto Prefectural University Of Medicine
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Hattori Takanori
Department Of Applied Chemistry Faculty Of Science & Technology Kinki University
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Fujita Setsuya
Department Of Dynamic Pathology Research Institute For Neurological Diseases And Geriatrics Kyoto Pr
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Fukuda Masaru
Department Of Anatomy Kyorin University School Of Medicine
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FUKUDA MASARU
Department of Pathology, Kyoto Prefectural University of Medicine
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