Multiple inflammatory cytokine-productive ThyL-6 cell line established from a patient with thymic carcinoma

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概要

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• Thymic epithelial cells can produce many kinds of cytokines, and interleukin (IL)-6-producing thymic carcinoma cases have been reported. However, a cytokine-producing human thymic tumor cell line has not previously been established. In this paper, we report a novel, multiple inflammatory cytokine-productive cell line that was established from a patient with thymic carcinoma. This cell line, designated ThyL-6, positively expressed epithelial membrane antigen, cytokeratins, vimentin intermediate filament and CD5, although hematological markers were not present in the cells. Cytokine antibody array analysis showed that the cells secreted several cytokines including IL-1α, IL-6, IL-8, RANTES, soluble TNFα-receptor 1, VEGF and CTLA into the culture medium. The addition of ThyL-6-cultured supernatant supported the growth of human myeloma ILKM-3 cells, which require the presence of IL-6 in the culture medium for the maintenance of cell growth, suggesting that the secreted IL-6 from ThyL-6 cells was biologically active. Chromosome analysis demonstrated that ThyL-6 cells had complex karyotype anomalies, including der(16)t(1;16); the latter has been recognized in thymic squamous cell carcinoma and thymic sarcomatoid carcinoma cases, as well as in several other kinds of malignancies. Heterotransplantation of the cells into nude mice showed tumorigenesis with neutrophil infiltration and liquefactive necrosis. These findings suggest that ThyL-6 cells will provide us with a new experimental tool for investigating not only the pathogenesis, biological behavior, chromo-somal analysis and therapeutic reagents of human thymic carcinoma, but also for studying cytokine-chemokine network systems.

• 日本癌学会の論文
• 2008-09-10

著者

• Ueda Takanori

  福井大学 血液腫瘍内科学

• Ueda T

  Department Of Hematology And Oncology University Of Fukui

• Ueda T

  Department Of Urology Chiba Cancer Center

• Ueda T

  First Department Of Internal Medicine University Of Fukui

• INAI Kunihiro

  Divisions of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences,

• UEDA Takanori

  Divisions of Hematology and Oncology, Department of General Medicine, Faculty of Medical Sciences, U

• TAKAGI Kazutaka

  Division of Hematology and Oncology, Department of General Medicine, Faculty of Medical Sciences, Un

• TAKIMOTO Nobuo

  Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, U

• OKADA Hiromi

  Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, U

• IMAMURA Yoshiaki

  Division of Surgical Pathology, Faculty of Medical Sciences, University of Fukui

• NAIKI Hironobu

  Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, U

• NORIKI Sakon

  Division of Tumor Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, Unive

• Noriki Sakon

  Division Of Tumor Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Universi

• Inai Kunihiro

  Division Of Molecular Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Univ

• Takimoto Nobuo

  Division Of Molecular Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Univ

• Naiki Hironobu

  Second Department Of Pathology University Of Fukui

• Naiki Hironobu

  Division Of Molecular Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Univ

• Noriki Sakon

  First Department Of Pathology Fukui Medical University

• Takagi Kazutaka

  Division Of Hematology And Oncology Department Of General Medicine Faculty Of Medical Sciences Unive

• Imamura Yoshiaki

  Division Of Surgical Pathology Faculty Of Medical Sciences University Of Fukui

• Imamura Y

  Tumour Pathology Fukui University Faculty Of Medical Sciences

• Ueda Takahiro

  First Department Of Internal Medicine Fukui Medical University Idarubicin Study Group

• Okada Hiromi

  Division Of Molecular Pathology Department Of Pathological Sciences Faculty Of Medical Sciences Univ

• Ueda Takanori

  Division Of Hematology And Oncology Faculty Of Medical Sciences University Of Fukui

• Ueda Takeshi

  Department Of Urology Chiba University Graduate School Of Medicine

• Naiki Hironobu

  Division Of Molecular Pathology Department Of General Medicine Faculty Of Medical Sciences University Of Fukui

• INAI Kunihiko

  Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui

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