Effects of Low-Dose X-Irradiation on the Development of the Mouse Cerebellar Cortex
スポンサーリンク
概要
- 論文の詳細を見る
We labeled proliferating cells of the cerebellum of 6-day-old mice with 5-bromo-2'-deoxyuridine (BrdU) followed by a single exposure to 0.5, 1 or 2 Gy of X-rays. We then studied the effects of low-dose irradiation on the migration and survival of granule neurons in the mouse cerebellum. The animals were killed at 4 days, or at 2, 4 or 6 weeks after irradiation. Brains were fixed and BrdU-labeled cells in the cerebella were immunohistochemically analyzed. BrdU was predominantly distributed in the superficial layer of the external granular layer soon after injection. Four days after irradiation with 0.5 or 1 Gy, labeled cells were mainly seen in the inner granular layer, which was also the case in non-irradiated mice. However, following 2 Gy irradiation BrdU was found not only in the inner granular layer, but also in the Purkinje cell layer. This distribution was also seen at 2 and 4-6 weeks after irradiation. In animals irradiated with 1 Gy 4-6 weeks after irradiation, the proportion of labeled cells present in the inner granular layer decreased, while labeled cells in the Purkinje cell layer increased. On the other hand, 0.5 Gy irradiation did not change the distribution of labeled cells, except that the proportion of labeled cells in the inner granular layer decreased at 2 weeks after irradiation. The number of labeled cells in the cerebellar cortex per unit area decreased with time and dose. These results suggest that 2 Gy irradiation induces a migratory delay, abnormal distribution, and cell death of the granule neurons of the mouse cerebellum.
- 日本放射線影響学会の論文
著者
-
FUSHIKI SHINJI
Department of Clinical Pathology, Kyoto Prefectural University of Medicine
-
Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
-
Fushiki Shinji
Department Of Clinical Pathology Kyoto Prefectural University Of Medicine:department Of Pathology Ky
-
HIROBE Tomohisa
Division of Biology, National Institute of Radiological Sciences
-
Ishikawa Yuji
Department Of Applied Biological Science School Of Agriculture Nagoya University
-
Ishikawa Yuji
Division of Biology, National Institute of Radiological Sciences
-
Hyodo-Taguchi Yasuko
Division of Biology, National Institute of Radiological Sciences
-
Kinoshita Chikako
Department of Pathology and Applied Neurobiology, Research Institute for Neurological Diseases and G
-
Fushiki S
Department Of Pathology And Applied Neurobiology Graduate School Of Medical Science Kyoto Prefectual
-
HYODO-TAGUCHI Yasuko
National Institute of Radiological Sciences
-
Hyodo‐taguchi Y
National Institute Of Radiological Sciences
-
Hyodo-taguchi Yasuko
Division Of Biology And Oncology National Institute Of Radiological Sciences
-
Hirobe T
National Inst. Radiological Sci. Chiba Jpn
-
Hirobe Tomohisa
Division Of Biology National Institute Of Radiological Sciences
-
Ishikawa Yuji
Division Of Biology National Institute Of Radiological Sciences
-
Kinoshita C
Kyoto Prefectural Univ. Medicine Kyoto Jpn
-
Kinoshita Chikako
Department Of Dynamic Pathology Research Institute For Neurological Diseases And Geriatrics Kyoto Pr
-
Ishikawa Yuji
Environmental Radiation Effects Research Group Research Center For Radiation Protection National Ins
-
HIROBE TOMOHISA
Division of Biology and Oncology, National Institute of Radiological Sciences
関連論文
- APPLICATION OF MICROWAVE IRRADIATION IN SURGICAL PATHOLOGY; IMPROVEMENT OF MICROSCOPIC-IMAGE OF CRYOSTAT SECTIONS AND EXPLORATION IN RAPID METALLIC STAININGS (Microwave Fixation in Histochemistry)
- Prenatal and Lactational Exposure to Bisphenol A in Mice Alters Expression of Genes Involved in Cortical Barrel Development without Morphological Changes
- Lysophospholipid Receptors Are Differentially Expressed in Rat Terminal Schwann Cells, As Revealed by a Single Cell RT-PCR and In Situ Hybridization
- Genetic Effects of Radiation in Atomic-bomb Survivors and Their Children : Past, Present and Future
- IA-07 Spatiotemporal patterns of expression of IGSF4 in developing mouse nervous system(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Prenatal Low-dose X-irradiation Affects Connexins 43 and 26 in Developing Mouse Neocortex
- Long-Term Organotypic Slice Culture of the Neonatal Mouse Liver
- The Proliferation and Differentiation of Neonatal Epidermal Melanocytes in F_1 Hairless Mice of HR-1×HR/De in Serum-Free Culture
- Rapid and Simple Method for Quantitative Evaluation of Neurocytotoxic Effects of Radiation on Developing Medaka Brain
- Apoptosis in neural tube during normal development of medaka
- Heritable malformations induced by X-irradiation in the medaka
- INDUCTION OF MUTATIONS BY ENU IN THE MEDAKA GERMLINE
- Stimulation of Insulin-dependent Glucose Transport and Lipogenesis in Isolated Rat Adipocytes by Trypsinized Soybean Glycinin Acidic Subunit A_
- Insulin-modulating Action of Glycinin Acidic Subunit A_ in Isolated Adipocytes Mimicking That of Bacitracin
- Stimulation of in Vitro Insulin Action by Glycinin Acidic Subunit A_(Biological Chemistry)
- Tissue culture sture study on neuronal migration in the rat cerebral cortex; effects of low dose radiation
- Contrasting Effects of Angiotensin Type 1 and 2 Receptors on Nitric Oxide Release under Pressure
- Effects of pre- and neonatal exposure to bisphenol A on murine brain development
- IA-01 Molecular changes in neurons of rat nucleus ambiguus after axotomy, as revealed by an in vivo fluorescence neuronal labeling combined with a single-cell RT-PCR(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Molecular Changes in Neurons of Rat Nucleus Ambiguus after Axotomy, As Revealed by a Novel Method of In Vivo Fluorescence Neuronal Labeling Combined with Single-cell RT-PCR
- The Effects of Heavy Ion Particles on the Developing Murine Cerebellum, with Special Reference to Cell Death
- Radiation-Induced Brain Cell Death Can Be Observed in Living Medaka Embryos
- Effects of Low-Dose X-Irradiation on the Development of the Mouse Cerebellar Cortex
- Effects of Radiation on the Development of Mouse Cerebellar Cortex.
- Effects of Continuous Low-Dose Prenatal Irradiation on Neuronal Migration in Mouse Cerebral Cortex
- Effects of Low-Dose Prenatal Irradiation on the Central Nervous System (IV)Neuronal Migration in Mouse Cerebral Cortex after Irradiation at Embryonic Day 13.
- Effects of Low-Dose Prenatal Irradiation on the Central Nervous System (II)Migration in Mouse Cerebral Cortex after Irradiation at Different Developmental Stages.
- Effects of Low-Dose Prenatal Irradiation on the Central Nervous System (l)Cell Migration in Mouse Cerebral Cortex after Irradiation.
- Notes on X-Ray Effects on the Testis of the Goby,Chasmichthys glosus
- Cell Population Change in Initiation of Spermatogenesis Following Exposure toHigh Temperature during Sexually Inactive Seasons in the Teleost,Oryzias latipes
- Establishment of Inbred Strains of the Medaka Oryzias latipes and the Usefulness of the Strains for Biomedical Research
- List of inbred strains of the medaka, Oryzias latipes, maintained in the Division of Biology, National Institute of Radiological Sciences
- Random genomic clones as a tool to construct genetic map in Japanese medaka, Orizias latipes
- Inbred strains of the medaka, Oryzias latipes(Development of Medaka Biology in Japan-Part I)
- Effects of Fractionated Whole-Body X-Irradiation on Cell Proliferation in theIntestine of the Goldfish,Carassius auratus
- Cryopreservation of Medaka Spermatozoa
- Identification of the Core Protein Carrying the Tn Antigen in Mouse Brain: Specific Expression on Syndecan-3
- Changes in Nitric Oxide Synthas-Containing Neurons in the Brain of Thiamine-Deficient Mice
- Estrogen and Non-Feminizing Estrogen for Alzheimer's Disease
- Heritable malformations induced by X-irradiation and ENU in the medaka
- Dose-Response and Large Relative Biological Effectiveness of Fast Neutrons with Regard to Mouse Fetal Cerebral Neuron Apoptosis
- Postnatal Changes in Mice Exposed In Utero to Fast Neutrons
- The Expression of p63 during Epidermal Remodeling in Psoriasis
- Proliferative Capacity Persists Most Tenaciously in the Murine Proximal Rostral Migratory Stream
- Histopathological Changes in the Detrusor Muscle Relation to Bladder Hypertrophy in BPH Patients
- Comparison of Cell Kinetics between the Boundary and the Interboundary Areas during Hindbrain Segmentation in the Chick Embryo
- Age-Related Changes of the Argyrophilic Nucleolar Organizer Regions in Mouse Neocortical Neurons
- An autopsy case of bathtub drowning in epilepsy
- A-5 A combined staining method for argyrophilic nucleolar organizer regions and for glial fibrillary acidic protein in astrocytes of human brain
- Recessive lethal mutants of the medaka (Oryzias latipes) maintained in the Division of Biology, National Institute of Radiological Sciences
- A brain atlas of a wild-type inbred strain of the medaka, Oryzias latipes(A Brain Atlas of Medaka)
- Embryonic development of the medaka brain(Development of Medaka Biology in Japan-Part II)
- P-50 Retrograde labeling of rat vagal neurons innervating lung and stomach by the carbocyanine dye DiI
- WS2-4 Cell adhesion molecule L1 (L1-CAM) and L1-linked developmental disorders of the nervous system(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Innervation of lateral line system in the medaka, Oryzias latipes
- Matrix Cells Dissociated from the Mouse Forebrain Vesicle Reaggregate to Form Neural Tube-like Organoid Structures in vitro
- A recessive lethal mutation, tb, that bends the midbrain region of the neural tube in the early embryo of the medaka
- Cell-to-cell Interactions during Development of the Neocortex, with Special Reference to Neuronal Migration
- Prenatal and lactational exposure to low-doses of bisphenol A alters adult mice behavior
- Two Japanese CADASIL Families Exhibiting Notch3 Mutation R75P Not Involving Cysteine Residue