Stimulation of Insulin Action and Stabilization of Cell Membrane in 3T3-L1 Cells by Glycinin Acidic Subunit A_<1a>

概要

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Glycinin acidic subunit A_<1a> and its trypsinized product (A_<1a>/Try) were found to potentiate insulin-mediated antilipolysis antagonized to isoproterenol in 3T3-L1 preadipocytes and adipocytes. A_<1a> and A_<1a>/Try protected 3T3-L1 preadipocytes from the cytotoxic action of trypan blue. Furthermore, the oxygen consumption in rat liver mitochondria that was accelerated during incubation, which implies an increase in the permeability of the inner membrane, was suppressed by the addition of A_<1a> and A_<1a>/Try. On the other hand, the chymotryptic product of A_<1a> did not show such effects. These results suggest that specific amino acid alignment is required for the functions of A_<1a> and that the insulin-stimulating action of A_<1a> and A_<1a>/Try is closely correlated with their stabilizing effect on cell membranes.
社団法人日本農芸化学会の論文
1995-08-23