Significant Expression of Functional Human Type 1 Mitochondrial ADP/ATP Carrier in Yeast Mitochondria
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概要
- 論文の詳細を見る
As a first step to characterize the unknown functional properties of the human mitochondrial ADP/ATP carrier(AAC), we tried to express human type 1 AAC(hAAC1)in Saccharomyces cerevisiae. Expression of hAAC1 in yeast mitochondrial membrene was very low, although its transcript level was high. Its expression was improved greatly by replacement of its N-terminal region with the corresponding region of yeast type 2 AAC(yAAC2), as observed with the bovine type 1 AAC(bAAC1). This chimeric hAAC1 showed similar ADP transport activity to that of chimeric bAAC1, corresponding to the transport activity of bAAC1 in bovine heart mitochondria. These results suggested that the N-terminal region of yAAC2 is important for expression of the mammalian carriers in yeast mitochondria. Using the present expression system, studies on the functional properties of the human AAC isoforms in relation to their structures are now possible.
- 公益社団法人日本薬学会の論文
- 2001-06-01
著者
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寺田 弘
東京理科大学薬学部
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Terada H
Nagoya Univ. Nagoya Jpn
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Hatanaka Tomomi
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Terada H
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
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Shinohara Yasuo
Institute For Genome Research University Of Tokushima
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Hashimoto Masahito
Department Of Chemistry Graduate School Of Science Osaka University
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HASHIMOTO Mitsuru
Graduate School of Pharmaceutical Sciences, Kumamoto University
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MAJIMA Eiji
APRO Life Science Institute, Inc.
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HATANAKA Takashi
Facluty of Pharmaceutical Sciences, University of Tokushima
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TAKEMOTO Yukiyo
Facluty of Pharmaceutical Sciences, University of Tokushima
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HASHIMOTO Mitsuru
Facluty of Pharmaceutical Sciences, University of Tokushima
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MAJIMA Eiji
Facluty of Pharmaceutical Sciences, University of Tokushima
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SHINOHARA Yasuo
Facluty of Pharmaceutical Sciences, University of Tokushima
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TERADA Hiroshi
Facluty of Pharmaceutical Sciences, University of Tokushima
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Majima Eiji
Apro Life Science Institute Inc.
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Takemoto Yukiyo
Facluty Of Pharmaceutical Sciences University Of Tokushima
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Hashimoto Mitsuru
Graduate School Of Pharmaceutical Sciences Kumamoto University
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寺田 弘
Fac. Of Pharmaceutical Sciences Tokyo Univ. Of Sci.
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Majima E
Facluty of Pharmaceutical Sciences, University of Tokushima
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Shinohara Y
Facluty of Pharmaceutical Sciences, University of Tokushima
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Terada H
Facluty of Pharmaceutical Sciences, University of Tokushima
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