Characterization of Δ22-Desaturation in Ergosterol Biosynthesis of Yeast
スポンサーリンク
概要
- 論文の詳細を見る
Conversion of ergosta-5, 7-dien-3β-ol to ergosterol (ergosta-5, 7, 22-trien-3β-ol)-Δ22-desaturation-by microsomes of Saccharomyces cerevisiae was inhibited by CO. The inhibition was partially reversed by illumination with white light. The Δ22-desaturation was also inhibited by SKF 525A. In addition, the activity was inhibited by the antibodies against yeast NADPH-cytochrome P-450 reductase [EC 1.6.2.4]. These results together with our preceding reports [S. Hata, T. Nishino, M. Komori and H. Katsuki, Biochem. Biophys. Res. Commun., 103, 272 (1981) and S. Hata, T. Nishino, H. Katsuki, Y. Aoyama and Y. Yoshida, Biochem. Biophys. Res. Commun., 116, 162 (1983)] suggest the contribution of a cytochrome P-450-containing monooxygenase system to the Δ22-desaturation, and a cytochrome P-450 (P-45022DS) may be responsible for the oxidative desaturation as the terminal enzyme. When semi-anaerobically-grown yeast cells were aerobically adapted, the Δ22-desaturation activity increased together with the increase in the cellular sterol content. The increment of Δ22-desaturation activity was inhibited by cycloheximide, indicating that the increase in the Δ22-desaturation activity was due to de novo synthesis of the enzyme protein(s). However, NADPH-cytochrome P-450 reductase, which is another component of the reaction, was nearly constant during the aerobic adaptation and was not affected by the cycloheximide. These results suggest that the component responsible for the induction of Δ22-desaturation activity is P-45022DS.
- 社団法人 日本農芸化学会の論文
著者
-
Yoshida Yuzo
Faculty Of Pharmaceutical Science Mukogawa Women's University
-
AOYAMA Yuri
Faculty of Engineering, Soka University
-
Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
-
Katsuki Hirohiko
Department of Chemistry, Faculty of Science, Kyoto University
-
Katsuki Hirohiko
Department Of Chemistry Faculty Of Science Kyoto University
-
HATA Shingo
Department of Chemistry, Faculty of Science, Kyoto University
-
HATA Shingo
Department of Chemistry for Materials, Graduate School of Engineering, Mie University
-
AOYAMA Yuri
Faculty of Pharmaceutical Science, Mukogawa University
関連論文
- Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1)(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Overexpression of an Archaeal Geranylgeranyl Diphosphate Synthase in Escherichia coli Cells
- Identification of Genes Affecting Lycopene Formation in Escherichia coli Transformed with Carotenoid Biosynthetic Genes: Candidates for Early Genes in Isoprenoid Biosynthesis
- Recognition of Allylic Substrates in Sulfolobus acidocaldarius Geranylgeranyl Diphosphate Synthase: Analysis Using Mutated Enzymes and Artificial Allylic Substrates^1
- Effects of Random Mutagenesis in a Putative Substrate-Binding Domain of Geranylgeranyl Diphosphate Synthase upon Intermediate Formation adn Substrate Specificity^1
- Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
- Identification of an inducible glucosyltransferase from Phytolacca americana L. cells that are capable of glucosylating capsaicin
- Effect of mutagenesis at the region upstream from the G(Q/E) motif of three types of geranylgeranyl diphosphate synthase on product chain-length(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Microbial Diversity in Biodegradation and Reutilization Processes of Garbage
- Molecular Cloning and Characterization of a Thermostable Carboxylesterase from an Archaeon, Sulfolobus shibatae DSM5389 : Non-Linear Kinetic Behavior of a Hormone-Sensitive Lipase Family Enzyme(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Molecular Biological Analysis of Microflora in a Garbage Treatment Process under Thermoacidophilic Conditions(ENVIRONMENTAL BIOTECHNOLOGY)
- Deciphering the Molecular Basis of the Broad Substrate Specificity of α-Glucosidase from Bacillus sp. SAM1606
- Deletion and Insertion of a 192-Residue Peptide in the Active-Site Domain of Glycosyl Hydrolase Family-2 β-Galactosidases
- Novel Sugar Phosphotransferase System Applicable to the Efficient Labeling of the Compounds Synthesized via the Non-Mevalonate Pathway in Escherichia coli
- An Active-Site Mutation Causes Enhanced Reactivity and Altered Regiospecificity of Transglucosylation Catalyzed by the Bacillus sp.SAM1606 α-Glucosidase
- Zinc-Finger Genes That Specifically Express in Pistil Secretory Tissues of Petunia
- Primary Structure and Catalytic Properties of a Cold-active Esterase from a Psychrotroph, Acinetobacter sp. Strain No.6. Isolated from Siberian Soil(Biochemistry & Molecular Biology)
- Cold-Active Lipolytic Activity of Psychrotrophic Acinetobacter sp. Strain No.6
- Gene Cloning and Overexpression of a Geranylgeranyl Diphosphate Synthase of an Extremely Thermophilic Bacterium, Thermus thermophilus
- Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin
- Identification of a cDNA Encoding Malonyl-Coenzyme A: Anthocyanidin 3-O-Glucoside 6"-O- Malonyltransferase from Cineraria (Senecio cruentus) Flowers
- PD28 FORMATION OF 12-HYDROXYSQUALENE CATALYZED BY SQUALENE SYNTHETASE
- Substrate Specificities of Several Prenyl Chain Elongating Enzymes with Respect to 4-Methyl-4-pentenyl Diphosphate
- An Artificial Substrate for the Thermostable Farnesyl Diphosphate Synthase from Bacillus stearothermophilus
- Mutational Analysis of the Role of His452 of Saccharopolyspora rectivirgula β-Galactosidase
- Effects of Y132H and F145L Substitutions on the Activity, Azole Resistance and Spectral Properties of Candida albicans Sterol 14-Demethylase P450 (CYP51) : A Live Example Showing the Selection of Altered P450 through Interaction with Environmental Compoun
- Insulin Is the Essential Factor Maintaining the Constitutive Expression of Hepatic Sterol 14-Demethylase P450(CYP51)
- Hopanoid Biosynthesis of Zymomonas mobilis(Microbiology & Fermentation Industry)
- Biochemical characterization and mutational studies of a chalcone synthase from yellow snapdragon (Antirrhinum majus) flowers
- Construction of a Plasmid for High Level Expression of Escherichia coli Phospho-enolpyruvate Carboxylase
- STUDIES ON THE RELATIONSHIP BETWEEN THE SPIN-STATE AND THE HEME ENVIRONMENT OF FERRIC CYTOCHROME P-450
- Thermostable Collagenolytic Activity of a Novel Thermophilic Isolate, Bacillus sp.Strain NTAP-1
- Phosphoenolpyruvate Carboxylase of Eschericia coli--Hydrophobic Chromatography Using Specific Elution with Allosteric Inhibitor
- Substrate Specificities of Wild and Mutated Farnesyl Diphosphate Synthases from Bacillus Stearothermophilus with Artificial Substrates
- Significance of Asn-77 and Trp-78 in the Catalytic Function of Undecaprenyl Diphosphate Synthase of Micrococcus luteus B-P 26^1
- Short-Chain Prenyl Diphosphate Synthase That Condenses Isopentenyl Diphosphate with Dimethylallyl Diphosphate in ispA Null Escherichia coll Strain Lacking Farnesyl Diphosphate Synthase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Disruption of the Structural Gene for Farnesyl Diphosphate Synthase in Escherichia coli
- EFFECTS OF BUTHIOBATE, A FUNGICIDE, ON CYTOCHROME P-450 OF RAT LIVER MICROSOMES
- Effects of Elevated Expression of the CYP51 (P450_) Gene on the Sterol Contents of Saccharomyces cerevisiae
- Identification and Characterization of Geranylgeraniol Kinase and Geranylgeranyl Phosphate Kinase from the Archaebacterium Sulfolobus acidocaldarius^1
- INTERACTION OF ANTIFUNGAL AGENTS WITH CYTOCHROME P-450 OF YEAST AND RAT LIVER MICROSOMES
- Protein Design of Geranyl Diphosphate Synthase. Strutural Features That Define the Product Specificities of Prenyltransferases
- Quantitative analyses of the behavior of exogenously added bacteria during an acidulocomposting process(ENVIRONMENTAL BIOTECHNOLOGY)
- Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Characterization of Δ22-Desaturation in Ergosterol Biosynthesis of Yeast
- N,N-Di- and N,N,C-Trialkylation of α-Sulfoximino Esters
- The Properties and Assignment of Isomers of 2,4-Dinitrophenylhydrazones of Some Aliphatic α-Keto Acids
- Studies on the Isomerization of 2,4-Dinitrophenylhydrazones of Some Aliphatic α-Keto Acids and the Preparation of Their Geometrical Isomers
- Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2
- Significance of Asn-77 and Trp-78 in the Catalytic Function of Undecaprenyl Diphosphate Synthase of Micrococcus luteus B-P 26.