Synthesis of Selenocystine and Selenohomocystine with O-Acetylhomoserine Sulfhydrylase
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概要
- 論文の詳細を見る
We describe here the synthesis of selenium araino acids with O-acetylhomoserine Sulfhydrylase, partially purified from bakers yeast. The enzyme was found to catalyze the synthesis of L-selenocystine and L-selenohomocystine from Na2Se2 with the corresponding acetyl-derivatives of serine and homoserine, respectively. L-Serine-O-sulfate also serves as a substrate of the β-replacement reaction. Na2Se2 is less efficient as a substituent donor than the physiological substrate, NaHS, and inhibits the enzyme at high concentrations. Therefore, limited amounts of Na2Se2 were added to the reaction mixture to increase the yield (50 to 60%). This provides a facile method to produce optically active Selenocystine and Selenohomocystine.
- 社団法人 日本農芸化学会の論文
著者
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Esaki N
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute for Chemical Research, Kyoto University
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TANAKA Hidehiko
Institute for Chemical Research, Kyoto University
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CHOCAT Patrick
ELF-Bio recherches, la Grande Borde, Labege
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SODAT Kenji
Institute for Chemical Research, Kyoto University
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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