Mammalian Selenocysteine Lyase Is Involved in Selenoprotein Biosynthesis

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概要

• 論文の詳細を見る

• Selenocysteine lyase (SCL) catalyzes the decomposition of L-selenocysteine to yield L-alanine and selenium by acting exclusively on l-selenocysteine. The X-ray structural analysis of rat SCL has demonstrated how SCL discriminates L-selenocysteine from L-cysteine on the molecular basis. SCL has been proposed to function in the recycling of the micronutrient selenium from degraded selenoproteins containing selenocysteine residues, but the role of SCL in selenium metabolism in vivo remains unclear. We here demonstrate that the 75Se-labeling efficiency of selenoproteins with 75Se-labeled selenoprotein P (Sepp1) as a selenium source was decreased in HeLa cells transfected with SCL siRNA as compared to the cells transfected with control siRNA. Immunocytochemical analyses showed high SCL expression in kidney and liver cells, where selenocysteine is recovered from selenoproteins. Mature testes of mice exhibited a specific staining pattern of SCL in spermatids that actively produce selenoproteins. However, SCL was weakly expressed in Sertoli cells, which receive Sepp1 and supply selenium to germ cells. These demonstrate that SCL occurs in the cells requiring selenoproteins, probably to recycle selenium derived from selenoproteins such as Sepp1.

• 財団法人 学会誌刊行センターの論文

著者

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• Kurokawa Suguru

  Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Takehashi Masanori

  Institute For Chemical Research Kyoto University

• Kato S

  Institute For Chemical Research Kyoto University

• Kurihara Tatsuo

  Institute For Chemical Research Kyoto University

• TAKEHASHI Masanori

  Faculty of Pharmacy, Osaka Ohtani University

• TANAKA Hiromitsu

  Faculty of Pharmaceutical Sciences, Nagasaki International University

• MIHARA Hisaaki

  College of Life Sciences, Ritsumeikan University

• TANAKA Seigo

  Faculty of Pharmacy, Osaka Ohtani University

• HILL Kristina

  Department of Medicine, Vanderbilt University School of Medicine

• BURK Raymond

  Department of Medicine, Vanderbilt University School of Medicine

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