IL-10 Augments Antibody Production in in Vitro Immunized Lymphocytes by Inducing a Th2-Type Response and B Cell Maturation
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概要
- 論文の詳細を見る
An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L-Leucine methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon antigen stimulation in the presence of IL-2, IL-4, and muramyl dipeptide. In the course of our studies, we have found that IL-10 added at the antigen sensitization significantly augmented antibody production level from the LLME-treated PBL. In the present study, we tried to demonstrate the role of IL-10 in the augmentation of antibody production in an IVI protocol by clarifying the cytokine expression profiles in CD4+ and CD8+ T cells. The results showed that IL-10 skewed the Th1/Th2 balance to Th2-type responses by suppressing Th1-type cytokine production and augmenting Th2-type cytokine production in CD4+ and CD8+ T cells, as well as in CD19+ B cells. Furthermore, IL-10 augmented the expression of CD38, an antigen marker of plasma cells, on B cells, which clearly indicates that IL-10 promoted differentiation and maturation of B cells in an IVI protocol. These results indicate that IL-10 plays an important role in setting the cellular milieu to produce antibodies in an IVI protocol.
- 2004-11-23
著者
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Tamura Takashi
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Osada K
Yakult Central Inst. Tokyo Jpn
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Aiba Yoshihiro
Graduate School Of Systems Life Sciences Faculty Of Agriculture Kyushu University
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Osada Kazuhiro
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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Osada Kazuhiro
Department Of Chemistry Science University Of Tokyo
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Katakura Y
Fac. Of Agriculture Kyushu Univ.
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KATAKURA Yoshinori
Department of Genetic Resources Technology, Faculty of Agriculture
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YAMASHITA Makiko
Department of Genetic Resources Technology, Faculty of Agriculture
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SHIRAHATA Sanetaka
Department of Genetic Resources Technology, Faculty of Agriculture
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NISHIKAWA Ryuhei
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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MATSUMOTO Shin-ei
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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AIBA Yoshihiro
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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TERUYA Kiichiro
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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XU Qianghua
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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FANG Shengguo
College of Life Sciences, Zhejiang University
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Osada Kazuhiro
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Xu Qianghua
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Fang Shengguo
College Of Life Sciences Zhejiang University
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Teruya Kiichiro
Fac. Of Agriculture Kyushu Univ.
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Shirahata Sanetaka
Graduate School Of Systems Life Sciences Kyushu University:department Of Genetic Resources Technolog
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Shirahata S
Fac. Of Agriculture Kyushu Univ.
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Tamura Takashi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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Yamashita Makiko
Fac. Of Agriculture Kyushu Univ.
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Katakura Yoshinori
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Tsuchiya T
Tsukuba Safety Assessment Laboratories Banyu Pharmaceutical Co. Ltd.
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Katakura Yoshinori
Dep. Of Genetic Resources Technol. Fac. Of Agriculture Kyushu Univ.
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Aiba Yoshihiro
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Tamura Takashi
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Maeyama Yoshiaki
R & D Division Nippon Luna Inc.
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Nishikawa Ryuhei
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Tsuchiya Toshie
Department Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Yamashita Makiko
R & D Division Nippon Luna Inc.
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SHIRAHATA Sanetaka
Department of Food and Nutrition, Shokei Junior College
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Shirahata Sanetaka
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
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Teruya Kiichiro
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
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