Establishment of a Novel Method of Screening Anti-Allergic Lactic Acid Bacteria

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概要

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• In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.

• 2011-05-23

著者

• Katakura Y

  Fac. Of Agriculture Kyushu Univ.

• TERUYA Kiichiro

  Graduate School of Systems Life Sciences, Kyushu University

• KATAKURA Yoshinori

  Graduate School of Systems Life Sciences, Kyushu University

• Morimatsu Fumiki

  R&d Center Nippon Meat Packers Inc.

• Teruya Kiichiro

  Fac. Of Agriculture Kyushu Univ.

• MORIMATSU Fumiki

  R&D Center, Nippon Meat Packers, Inc.

• Yamashita Makiko

  Fac. Of Agriculture Kyushu Univ.

• Yamashita Makiko

  Faculty Of Agriculture Kyushu University

• Katakura Yoshinori

  Dep. Of Genetic Resources Technol. Fac. Of Agriculture Kyushu Univ.

• Hasegawa Takanori

  Research And Development Center Nippon Meat Packers Inc.

• Maeyama Yoshiaki

  R & D Division Nippon Luna Inc.

• Morimatsu Fumiki

  Res. And Dev. Center Nippon Meat Packers Inc.

• KADOOKA Keishi

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• IMAHAYASHI Asuka

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• KOISO Ayaka

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• MATSUMOTO Takashi

  R&D Center, Nippon Meat Packers, Inc.

• HASEGAWA Takanori

  R&D Center, Nippon Meat Packers, Inc.

• Katakura Yoshinori

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Teruya Kiichiro

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Kadooka Keishi

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Yamashita Makiko

  R & D Division Nippon Luna Inc.

• Imahayashi Asuka

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Koiso Ayaka

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Matsumoto Takashi

  R&d Center Nippon Meat Packers Inc.

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