Selenite Reduction by the Thioredoxin System: Kinetics and Identification of Protein-Bound Selenide
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概要
- 論文の詳細を見る
Selenite (SeO32−) assimilation into a bacterial selenoprotein depends on thioredoxin (trx) reductase in Esherichia coli, but the molecular mechanism has not been elucidated. The mineral-oil overlay method made it possible to carry out anaerobic enzyme assay, which demonstrated an initial lag-phase followed by time-dependent steady NADPH consumption with a positive cooperativity toward selenite and trx. SDS-PAGE/autoradiography using 75Se-labeled selenite as substrate revealed the formation of trx-bound selenium in the reaction mixture. The protein-bound selenium has metabolic significance in being stabilized in the divalent state, and it also produced the selenopersulfide (-S-SeH) form by the catalysis of E. coli trx reductase (TrxB).
著者
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Esaki Nobuyoshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Tamura T
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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MIHARA Hisaaki
Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University
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Tamura Takashi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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SATO Kumi
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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KOMORI Kentaro
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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IMAI Takeshi
Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University
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KUWAHARA Mitsuhiko
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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OKUGOCHI Takahiro
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Kawamura Kentaro
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Kuwahara Mitsuhiko
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Sato Kumi
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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