Identification of the Product of Sporulation Gene spoOB in Bacillus subtilis
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概要
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A 1.4-megadalton EcoRI restriction fragment carrying Bacillus subtilis speculation gene spoOB was cloned from the specialized transducing phage, ø105spoOB, into a unique EcoRI site of plasmid vector pUBHO, and four plasmids having a deletion in the 1.4-megadalton EcoRI fragment were constructed. Analysis of the polypeptides synthesized in B. subtilis minicells harboring these plasmids and the sporulation ability of strain UOT0436 (spoOB136 recE4) harboring these plasmids showed that the spoOB gene product is a polypeptide of 24, 000 daltons. Two-dimensional polyacrylamide gel analysis showed that the isoelectric point of this protein is almost neutral.
- 社団法人 日本農芸化学会の論文
著者
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Oda Masanao
Department Of Applied Biochemistry Hiroshima University
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Nomi Ryosaku
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Saito Hiuga
Institute Of Applied Microbiology University Of Tokyo
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Kobayashi Yasuo
Department Of Anesthesia Asahikawa City Hospital
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YAMADA Kazunori
Department of Applied Biochemistry, Hiroshima University
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SHIMOTSU Hidenori
Institute of Applied Microbiology, The University of Tokyo
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