X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
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概要
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A bacterial two-component signal transduction system, WalK/WalR, is essential to the cell viability of Gram-positive bacteria and is therefore a potential target for the development of a new class of antibiotics. We have solved the X-ray crystal structure of the DNA-binding domain of the response regulator WalR (WalRc) from a Gram-positive pathogen Staphylococcus aureus, currently causing serious problems in public health through the acquisition of multi-drug resistance. The structure contains a winged helix-turn-helix motif and closely resembles those of WalRs of Bacillus subtilis and Enterococcus faecalis, and also that of PhoB of Escherichia coli. Gel mobility shift assays with mutant WalRs revealed specific interactions of WalR with the target DNA, as elaborated by in silico modeling of the WalRc-DNA complex.
- 社団法人 日本農芸化学会の論文
著者
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DOI Akihiro
Department of Physics, Faculty of Science, Yamaguchi University
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TANIZAWA Katsuyuki
Institute for Chemical Research, Kyoto University
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Okajima Toshihide
Institute of Scientific and Industrial Research, Osaka University
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Utsumi Ryutaro
Department of Agricultural Chemistry, Kinki University
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Gotoh Yasuhiro
Department of Bioscience, Graduate School of Agriculture, Kinki University
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Gotoh Yasuhiro
Department Of Bioscience Graduate School Of Agriculture Kinki University
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