Efficient Production of Recombinant Human (Pro)renin Utilizing a Decahistidine Tag Attached at the C-Terminus
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概要
- 論文の詳細を見る
Human prorenin attached by a decahistidine tag at the C-terminus was produced in Chinese hamster ovary cells. The tagged protein secreted into the culture medium was in the inactive prorenin form, and was activated to mature renin by proteolytic removal of its prosegment by trypsin in the same manner as native prorenin. The tagged (pro)renin was efficiently purified by metal-chelate affinity chromatography. The enzymatic properties of mature renin carrying the tag were similar to native renin. These results indicate that the introduction of a decahistidine tag at the C-terminus does not interfere with either the correct folding of prorenin or the catalytic activity of mature renin.
- 社団法人 日本農芸化学会の論文
著者
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Suzuki Fumiaki
Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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Nakamura Yukio
Laboratory Of Organic Chemistry Faculty Of Technology Kanagawa University
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IWATA Hideyuki
United Graduate School of Agricultural Science, Gifu University
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NAKAGAWA Tsutomu
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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NISHIUCHI Kazuhiro
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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AKAKI Jyunji
Laboratory of Applied Biochemistry, Faculty of Applied Biological Science, Gifu University
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SATOU Ryousuke
United Graduate School of Agricultural Science, Gifu University
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