Display of the human (pro)renin receptor on Bombyx mori nucleopolyhedrovirus (BmNPV) particles using Bm cells(MEDICAL BIOTECHNOLOGY)
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概要
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The human (pro)renin receptor (hPRR) was displayed on the surface of Bombyx mori nucleopolyhedrovirus (BmNPV) with and without fusion to glycoprotein 64 (GP64) of the BmNPV. hPRR1 is a native hPRR with an additional FLAG peptide sequence inserted between the signal peptide and prorenin-binding domain. hPRR2 has the prorenin-binding domain inserted between amino acid residues ^<81>Asp and ^<82>Pro of GP64. hPRR4 has the prorenin-binding domain inserted in ^<81>Asp and ^<320>Met of partially deleted GP64. Incorporation of hPRR was confirmed in recombinant BmNPV (rBmNPV) but not in cysteine protease-deleted rBmNPV. hPRR1 was observed in ER, but hPRR2 and hPRR4 were observed around the endoplasmic reticulum (ER) and in its periphery. rBmNPV-hPRR1 and -hPRR2, carrying hPRR1 and hPRR2 respectively, showed binding affinity to human renin, but rBmNPV-hPRR4 did not. The presence of hPRR4 of rBmNPV-hPRR4 was confirmed in western blotting under nonreducing conditions, suggesting that although hPRR4 was incorporated in rBmNPV-hPRR4, it behaved as a non-functional aggregate. This rBmNPV display system can also be used for analyzing a ligand-receptor interaction.
著者
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Kato Tatsuya
Laboratory of Biotechnology, Faculty of Agriculture, Shizuoka University
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Suzuki Fumiaki
Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University
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Park Enoch
Laboratory Of Biotechnology Integrated Bioscience Section Graduate School Of Science And Technology
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Suzuki Fumiaki
Laboratory Of Animal Biochemistry Faculty Of Applied Biological Sciences Gifu University
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Kato Tatsuya
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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