Enhancement of ε-Polylysine Production by Streptomyces albulus Strain 410 Using pH Control
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概要
- 論文の詳細を見る
The enhancement of ε-poly-L-lysine (ε-PL) production by Streptomyces albulus strain no.410 (S410) by means of a pH control strategy was investigated. S140 cells produce ε-PL at a high concentration if the culture pH remains at about 4.0; however, if it shifts to higher than 4.0, the accumulated ε-PL is depolymerized. We therefore suggest a pH control strategy for cell growth and ε-PL production aimed at increasing the amount of ε-PL produced. The cultivation was divided into two control phases. In phase I, cell growth was accelerated by maintaining the pH at higher than 5.0; in phase II, ε-PL production was increased by maintaining the pH at about 4.0. To avoid an increase in the pH during phase II as a result of glucose depletion, the glucose concentration was kept at around 10 g/l by glucose feeding. This control strategy enhanced the production of ε-PL to 48.3 g/l from 5.7 g/l in the case of batch culture.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University:integrate
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OKABE MITSUYASU
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Kahar P
Gifu Univ. Gifu Jpn
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Kahar Prihardi
United Graduate School Of Agricultural Science Gifu University
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Park Enoch
Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University
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Hiraki Jun
Yokohama Research Centers Chisso Cooperation
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Okabe Mitsuyasu
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Okabe Mitsuyasu
Mercian Co. Central Research Laboratories
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Okabe Mitsuyasu
Biotechnology Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Univer
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Okabe Mitsuyasu
Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University
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Okabe Mitsuyasu
Biochemical Engineering Laboratory Department Of Applied Biological Chemistry Faculty Of Agriculture
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Okabe Mitsuyasu
(present Address)department Of Applied Biochemistry Faculty Of Agriculture Shizuoka University
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IWATA TOSHIHARU
Yokohama Research Centers Chisso Cooperation
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Iwata T
Yokohama Research Centers Chisso Cooperation
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Okabe M
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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