Effect of Consumed Carbon to Nitrogen Ratio on Mycelial Morphology and Arachidonic Acid Production in Cultures of Mortierella alpina
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概要
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The influence of the consumed carbon to nitrogen(C/N)ratio on arachidonic acid(AA)production and mycelial morphology was investigated in cultures of Mortierella alpina using shake flasks and a fermentor. The consumed C/N ratio was varied from 5 to 32 under the condition that the total initial amount of carbon and nitrogen sources was 50 g/l. Cellular yield increased markedly at C/N ratios below 7 ; carbon utilization was switched from cellular growth to lipid biosynthesis in the C/N ratio range of 7-15 ; lipid biosynthesis was most active when the C/N ratio was in the range of 15-32. However, for C/N ratios higher than 15, the mycelial concentration decreased due to nitrogen limitation but the lipid yield still increased. In the presence of excess nitrogen, the biomass concentration depended on the amount of the nitrogen source, but the AA yield was inversely related to this. On the other hand, in the presence of excess carbon, the fatty acid concentration increased with carbon source concentration but the AA concentration remained constant. From the viewpoint of AA production, the optimum C/N ratio was in the range of 15 to 20 with a balance between the amounts of carbon and nitrogen sources. When an enriched medium was used at a fixed C/N ratio of 20, the cellular and AA concentrations were shown to be proportional to the total concentrations of carbon and nitrogen sources in both flasks and the fermentor. The whole pellet size and width of pellet annular regions did not change with increasing C/N ratio for C/N ratios below 20 in the flask cultures. However, when the C/N ratio was higher than 20, these sizes increased in proportion to the C/N ratio.
- 社団法人日本生物工学会の論文
- 2001-04-25
著者
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University:integrate
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Park E
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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東山 堅一
サントリー
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東山 堅一
サントリー健康科学研
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東山 堅一
サントリー株式会社
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KOIKE YASUHISA
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi
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CAI HONG
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi
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HIGASHIYAMA KENICHI
Institute for Fundamental Research, Suntory Ltd.
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FUJIKAWA SHIGEAKI
Institute for Fundamental Research, Suntory Ltd.
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Cai Hong
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Koike Y
Nagoya Univ. Nagoya Jpn
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Koike Yoichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Higashiyama Kenichi
Institute For Fundamental Research Suntory Ltd.
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Fujikawa S
Institute For Fundamental Research Suntory Ltd.
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Fujikawa Shigeaki
Institute For Fundamental Research Suntory Ltd.
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