Molecular Design of Fluorescent Labeled Glycosides as Acceptor Substrates for Sialyltransferases

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概要

• 論文の詳細を見る

• A series of dansyl-labeled glycosides with di-, tetra-, and hexasaccharides carrying the terminal N-acetyllactosamine (LacNAc) sequence were synthesized as acceptor substrates for α2,6- and α2,3-sialyltransferases. As an alternative design, dansyl-labeled LacNAc glycoside carrying a long-spacer linked glycan was engineered by replacement of the LacNAc or lactose units with an alkyl chain. In addition, we designed a dansyl-labeled bi-antennary LacNAc glycoside as an N-linked oligosaccharide mimetic, such as asialo-α1-acid glycoprotein. The kinetic parameters for the transfer reaction of synthesized dansyl-labeled glycosides by sialyltransferases were determined by the fluorescent HPLC method. The catalytic efficiencies (Vmax/Km) of acceptor substrates carrying the terminal LacNAc sequence with various length glycans in the array for α2,6- and α2,3-sialyltransferases decreased in a glycan length-dependent manner. Furthermore, of the acceptor substrates tested, dansyl-labeled bi-antennary LacNAc glycoside displayed the most favorable Km value for α2,6- and α2,3-sialyltransferases.

• 2010-11-23

著者

• Park Enoch

  Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University:integrate

• Usui Taichi

  Science Of Biological Resource The United Graduate School Of Agricultural Science Gifu University

• Usui Taichi

  Department Of Agricultural Chemistry Faculty Of Agriculture Shizuoka University

• Murata Takeomi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Murata Takeomi

  Department Of Applied Biological Cemistry Faculty Of Agriculture Shizuoka University

• Murata Takeomi

  Department Of Biological Chemistry Faculty Of Agriculture Shizuoka University

• Murata T

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Usui T

  Dep. Of Applied Biological Chemistry Fac. Of Agriculture Shizuoka Univ.

• Park Enoch

  Department Of Bioscience Graduate School Of Science And Technology Shizuoka University

• Usui Taichi

  Department Of Bioscience Graduate School Of Science And Technology Shizuoka University

• Ogata Makoto

  Department Of Bioscience Graduate School Of Science And Technology Shizuoka University

• OBARA Takakiyo

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• CHUMA Yasushi

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• Chuma Yasushi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Obara Takakiyo

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Ogata Makoto

  Department of Bioscience, Graduate School of Science and Technology, Shizuoka University

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