Effect of Nitrogen Source on Mycelial Morphology and Arachidonic Acid Production in Cultures of Mortierella alpina

スポンサーリンク

概要

• 論文の詳細を見る

• The effects of nitrogen source on arachidonic acid(AA)production and morphological changes during the culture of Mortierella alpina were investigated using an image analysis stystem. When yeast extract, gluten meal, or corn steep liquor was used, a circular pellet morphology was obtained. However, when Pharmamedia, fish meal, or soybean meal was used, M.alpina formed radial filamentous mycelia. The radial filamentous area in the case of soybean meal was 75% of the total mycelial area. In a jar fermentor culture, M.alpina morphology varied with the cultivation period : (i)at 0-6h culture, the inoculated pellet-like mycelia were adapted to the new enviornment, (ii)at 6h-1d culture, filamentous mycelia grew exponentially which yielded a feather-like morphology, (iii)at 1-2d culture, the filamentous mycelia became disentangled as a result of the mechanical agitation ; consequently, the proportion of filamentous mycelia was increased, (iv)at 2-4d culture, mycelia showed stationary growth, but the AA concentration increased rapidly, and(v) at 4-6d culture, hyphae grew thick radially with the AA concentration continuing to increase gradually. In the case of the cultures with feather-like morphology obtained using soybean meal, the AA yield was 0.14 g/g dry cell weight, which was two times higher than that in cultures grown using yeast extract. These results suggest that the feather-like morphology of culture of M.alpina is suitable for AA production.

• 社団法人日本生物工学会の論文
• 1999-07-25

著者

• Park Enoch

  Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University:integrate

• Park E

  Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo

• OKABE MITSUYASU

  Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi

• Park Enoch

  Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo

• 東山 堅一

  サントリー

• 東山 堅一

  サントリー健康科学研

• 東山 堅一

  サントリー株式会社

• KOIKE YASUHISA

  Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi

• HIGASHIYAMA KENICHI

  Institute for Fundamental Research, Suntory Ltd.

• FUJIKAWA SHIGEAKI

  Institute for Fundamental Research, Suntory Ltd.

• Okabe Mitsuyasu

  Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo

• Okabe Mitsuyasu

  Mercian Co. Central Research Laboratories

• Okabe Mitsuyasu

  Biotechnology Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Univer

• Okabe Mitsuyasu

  Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University

• Okabe Mitsuyasu

  Biochemical Engineering Laboratory Department Of Applied Biological Chemistry Faculty Of Agriculture

• Okabe Mitsuyasu

  (present Address)department Of Applied Biochemistry Faculty Of Agriculture Shizuoka University

• Koike Y

  Nagoya Univ. Nagoya Jpn

• Koike Yoichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Okabe M

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Higashiyama Kenichi

  Institute For Fundamental Research Suntory Ltd.

• Fujikawa S

  Institute For Fundamental Research Suntory Ltd.

• Fujikawa Shigeaki

  Institute For Fundamental Research Suntory Ltd.

関連論文

• Binding Affinity of Full-length and Extracellular Domains of Recombinant Human (Pro)renin Receptor to Human Renin When Expressed in the Fat Body and Hemolymph of Silkworm Larvae.
• Human single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids
• 422. Mortierella alpinaによるアラキドン酸生産に及ぼす溶存酸素濃度の影響
• Improvement of Tylosin Production from Streptomyces fradiae Culture by Decreasing the Apparent Viscosity in an Air-Lift Bioreactor
• 920 Mortierella alpina 1S-4株の菌形態制御による高アラキドン酸生産の実現
• Oxidation of Rapeseed Oil in Waste Activated Bleaching Earth and Its Effect on Riboflavin Production in Culture of Ashbya gossypii
• Optimization of Tylosin Feeding Rate Profile in Production of Acetyl-Isovaleryl Tylosin(AIV)from Tylosin by Streptomyces thermotolerans YN554
• Effect of Soybean Oil on Oxygen Transfer in the Production of Tetracycline with an Airlift Bioreactor
• Enhancement of Yeast Fermentation by Addition of Oxygen Vectors in Air-Lift Bioreactor
• 3P-2135 Efficient Cellulase Production in the Culture of Acremonium cellulolyticus Utilizing Paper Sludge as Carbon Source
• Effect of Consumed Carbon to Nitrogen Ratio on Mycelial Morphology and Arachidonic Acid Production in Cultures of Mortierella alpina
• Effect of Nitrogen Source on Mycelial Morphology and Arachidonic Acid Production in Cultures of Mortierella alpina
• Lipase-catalyzed biodiesel production from waste activated bleaching earth as raw material in a pilot plant
• Repeated production of fatty acid methyl ester with activated bleaching earth in solvent-free system
• Efficient Production of Fatty Acid Methyl Ester from Waste Activated Bleaching Earth Using Diesel Oil as Organic Solvent(ENVIRONMENTAL BIOTECHNOLOGY)
• Image Analysis of Morphological Change during Arachidonic Acid Production by Mortierella alpina 1S-4
• 2I09-4 糸状菌Mortierella alpinaにおける菌糸形態形成へのアミノ酸の影響(生物化学工学,一般講演)
• 油脂の発酵生産と生産菌の形態変化
• 335 アラキドン酸生産菌Mortierella alpinaの菌糸形態形成のモニタリング(生物化学工学,一般講演)
• 425 フロースルーチャンバーによるアラキドン酸生産カビMortierella alpinaの菌糸形成の解析
• 糸状菌によるアラキドン酸含有油脂の発酵生産
• 油脂の発酵生産と生産菌の形態変化
• 1049 誘電計測による糸状菌Mortierella alpinaの菌体内油脂含量推定
• 1036 Mortierella alpinaによるアラキドン酸の生産及び菌糸形態における培地内C/N比の影響
• 919 Mortierella alpinaを用いたアラキドン酸の生産における菌糸形態の影響
• Increased Riboflavin Production from Activated Bleaching Earth by a Mutant Strain of Ashbya gossypii.
• Synthesis of sialoglycopolypeptide for potentially blocking Influenza virus infection using a rat alpha2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae
• Molecular Chaperone-Assisted Production of Human α-1,4-N-Acetylglucosaminyltransferase in Silkworm Larvae Using Recombinant BmNPV Bacmids
• Mycelial Pellet Intrastructure Visualization and Viability Prediction in a Culture of Streptomycese fradiae Using Confocal Scanning Laser Microscopy
• Change of Mycelial Morphology in Tylosin Production by Batch Culture of Streptomyces fradiae under Various Shear Conditions
• Comparison of the N-linked glycosylation of human β1,3-N-acetylglucosaminyltransferase 2 expressed in insect cells and silkworm larvae
• Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure
• Kojic Acid Production in an Airlift Bioreactor Using Partially Hydrolyzed Raw Corn Starch
• Identification of Sialyltransferases of Streptococcus agalactiae
• Molecular Characterization of a Novel β1, 3-Galactosyltransferase for Capsular Polysaccharide Synthesis by Streptococcus agalactiae Type Ib^1
• Putative Regulatory Gene for Polysaccharide Synthesis of Streptococcus agalactiae Type Ia
• Production of ε-Polylysine in an Airlift Bioreactor (ABR)
• Enhancement of ε-Polylysine Production by Streptomyces albulus Strain 410 Using pH Control
• Production of L-Lactic Acid from Corncob (BIOCHEMICAL ENGINEERING)
• 2C-AM4 糸状菌Mortierella alpinaの高濃度培養によるアラキドン酸含有油脂の生産(新たな機能性脂質の設計と生産,シンポジウム)
• 555 ハイブリドーマ培養における抗体生産に及ぼすグルタミン濃度の影響
• Kinetics of Soybean Oil Consumption and Cephamycin C Production in Culture of Streptomyces sp.Using Mineral Support
• Effects of Rapeseed Oil on Activity of Methylmalonyl-CoA Carboxyltransferase in Culture of Streptomyces fradiae
• Scale-Up of L-Lactic Acid Production by Mutant Strain Rhizopus sp. MK-96-1196 from 0.003m^3 to 5m^3 in Airlift Bioreactors(BIOCHEMICAL ENGINEERING)
• 2D10-5 Production of itaconic acid using Indonesia sago starch by Aspergillus terreus TN484-M1
• Enhanced Production of L-Lactic Acid by Ammonia-Tolerant Mutant Strain Rhizopus sp. MK-96-1196
• Optimization and Scale-Up of L-Lactic Acid Fermentation by Mutant Strain Rhizopus sp. MK-96-1196 in Airlift Bioreactors (BIOCHEMICAL ENGINEERING)
• Improvement of Production of Kojic Acid by a Mutant Strain Aspergillus oryzae, MK107-39
• L(+)-Lactic Acid Production by Repeated Batch Culture of Rhizopus oryzae in Air-Lift Bioreactor
• Efficient Itaconic Acid Production from Raw Corn Starch
• Enhanced Production of L(+)-Lactic Acid from Corn Starch in a Culture of Rhizopus oryzae Using an Air-Lift Bioreactor
• Optimization of Conditions for Conversion of Tylosin to a Novel Antibiotic, Acetyl-Isovaleryl Tylosin (AIV), by Streptomyces thermotolerans and Scale-Up to 200-Liter Pilot-Scale Fermentor
• Purification and Characterization of cis-Aconitic Acid Decarboxylase from Aspergillus terreus TN484-M1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Improvement of GFPuv-β3GnT2 Fusion Protein Production by Suppressing Protease in Baculovirus Expression System(Biochemistry & Molecular Biology)
• Angiogenesis and Inflammatory Cell Infiltration in Lumbar Disc Herniation
• Preferential Production of a Carbapenem Antibiotic, PS-5 by Dissolved Oxygen Controlled Fermentation
• Preferential and High-Yield Production of a Cephamycin C by Dissolved Oxygen Controlled Fermentation
• ものと人間の文化史138 麹(こうじ), 一島英治著, 四六判, 244頁, 2500円+税, 法政大学出版局
• 菌形態が物質生産に影響するのは本当か?(バイオミディア2000)
• Comparison of Neomycin Production from Streptomyces fradiae Cultivation Using Soybean Oil as the Sole Carbon Source in an Air-Lift Bioreactor and a Stirred-Tank Reactor
• Neomycin Production by Partial Immobilization Streptomyces fradiae on Cellulose Beads in an Air-Lift Bioreactor
• Itaconic Acid Production in an Air-Lift Biorector Using a Modified Draft Tube
• A Novel Process of Cyclodextrin Production by the Use of Specific Adsorbents : Part II. A New Reactor System for Selective Production of α-Cyclodextrin with Specific Adsorbent
• A Novel Process of Cyclodextrin Production by the Use of Specific Adsorbents : Part I. Screening of Specific Adsorbents
• Visualization of a Recombinant Gene Protein in the Baculovirus Expression Vector System Using Confocal Scanning Laser Microscopy
• Functional Expression of Streptococcal Galactosyltransferase in Baculovirus/Insect Cell Expression System(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Coliphage Derived Sialidase Preferentially Recognizes Nonreducing End of Polysialic Acid(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Breeding of Aspergillus terreus Mutant TN-484 for Itaconic Acid Production with High Yield
• Improvement of Cephamycin C Production by a Mutant Resistant to Linoleic Acid
• Molecular Design of Fluorescent Labeled Glycosides as Acceptor Substrates for Sialyltransferases
• Brilliant Skyblue Pigment Formation from Gardenia Fruits
• Genipin, a New Type of Protein Crosslinking Reagent from Gardenia Fruits(Microbiology & Fermentation Industry)
• Determing Factors of Mortality in the Elderly with Hip Fractures
• Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• BIOCONVERSION OF WASTE OFFICE PAPER TO L(+)-LACTIC ACID BY FILAMENTOUS FUNGUS RHIZOPUS ORYZAE
• REUTILIZATION OF VEGETABLE OILS: FROM WASTES TO RENEWABLE DIESEL FUEL
• Display of the human (pro)renin receptor on Bombyx mori nucleopolyhedrovirus (BmNPV) particles using Bm cells(MEDICAL BIOTECHNOLOGY)
• Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator
• 3Ap07 Potential in bioethanol production from waste paper sludge in pulp-based biorefinery(Japan-Korea Biomass Session)
• Tracking Neospora caninum parasites using chimera monoclonal antibodies against its surface antigen-related sequences (rNcSRS2)(CELL AND TISSUE ENGINEERING)

もっと見る 閉じる

スポンサーリンク