Increased Riboflavin Production from Activated Bleaching Earth by a Mutant Strain of Ashbya gossypii.
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概要
- 論文の詳細を見る
The production of riboflavin from vegetable oil was increased using a mutant strain of Ashbya gossypii. This mutant was generated by treating the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Riboflavin production was 10-fold higher in the mutant compared to the wild-type strain. The specific intracellular catalase activity after 3d of culture was 6-fold higher in the mutant than in the wild-type strain. For the mutant, riboflavin production in the presence of 40mM hydrogen peroxide was 16% less than that in the absence of hydrogen peroxide, whereas it was 56% less for the wild-type strain. The isocitrate lyase (ICL) activity of the mutant was 0.26mU/mg of protein during the active riboflavin production phase, which was 2.6-fold higher than the wild-type strain. These data indicate that the mutant utilizes the carbon flux from the TCA cycle to the glyoxylate cycle more efficiently than the wild-type strain, resulting in enhanced riboflavin production. This novel mutant has the potential to be of use for industrial-scale riboflavin production from waste-activated bleaching earth (ABE), thereby transforming a useless material into a valuable bioproduct.
- 社団法人日本生物工学会の論文
著者
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University:integrate
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Kato Tatsuya
Laboratory of Biotechnology, Faculty of Agriculture, Shizuoka University
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Itoh Yoko
Jst Innovation Satellite Shizuoka Japan Science And Technology Agency
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Park Enoch
Laboratory Of Biotechnology Integrated Bioscience Section Graduate School Of Science And Technology
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Park Enoch
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Kato Tatsuya
Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University
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Tajima Satoshi
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi
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Sugimoto Takashi
Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technolog
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Tajima Satoshi
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Kato Tatsuya
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Shizuoka University
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Kato Tatsuya
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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