Isolation of an oxalate-resistant Ashbya gossypii strain and its improved riboflavin production.
スポンサーリンク
概要
- 論文の詳細を見る
An oxalate-resistant strain of Ashbya gossypii was naturally isolated from spores grown on an oxalate-containing medium, and its medium was optimized to improve riboflavin production. Riboflavin production by the resistant strain was three-fold higher than that by the wild-type organism when grown in flask cultures. Medium optimization increased the riboflavin production by the resistant strain to 5 g l(-1), which was five-fold higher than that obtained by the wild-type strain. The productivity was reproduced in a 3-l bioreactor. During the early growth phase, the specific activity of isocitrate lyase in the oxalate-resistant strain was slightly higher than that in the wild-type strain. Proteomic analysis of the oxalate-resistant strain revealed that the expression of aldose reductase and cobalamin-independent methionine synthase decreased significantly. This is the first report that describes the natural isolation of a riboflavin producer using an antimetabolite-containing medium to enhance the riboflavin production level. This method should also be useful for improving the productivity of other bioproducts since it does not require any mutations or genetic modifications of the microorganism.
- Springerの論文
著者
-
Kato Tatsuya
Laboratory of Biotechnology, Faculty of Agriculture, Shizuoka University
-
Park Enoch
Laboratory Of Biotechnology Integrated Bioscience Section Graduate School Of Science And Technology
-
Kato Tatsuya
Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University
関連論文
- Binding Affinity of Full-length and Extracellular Domains of Recombinant Human (Pro)renin Receptor to Human Renin When Expressed in the Fat Body and Hemolymph of Silkworm Larvae.
- Human single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids
- 1Hp21 EFFICIENT SACCHARIFICATION OF PAPER SLUDGE BY A. cellulolyticus CELLULASE
- Expression and purification of human (pro)renin receptor in insect cells using baculovirus expression system
- Construction of a cysteine protease deficient Bombyx mori multiple nucleopolyhedrovirus bacmid and its application to improve expression of a fusion protein
- Enhanced production of secretory β1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence
- 3P-2135 Efficient Cellulase Production in the Culture of Acremonium cellulolyticus Utilizing Paper Sludge as Carbon Source
- Characterization of SEZ6L2 cell-surface protein as a novel prognostic marker for lung cancer
- Isolation of an oxalate-resistant Ashbya gossypii strain and its improved riboflavin production.
- Increased Riboflavin Production from Activated Bleaching Earth by a Mutant Strain of Ashbya gossypii.
- Synthesis of sialoglycopolypeptide for potentially blocking Influenza virus infection using a rat alpha2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae
- Molecular Chaperone-Assisted Production of Human α-1,4-N-Acetylglucosaminyltransferase in Silkworm Larvae Using Recombinant BmNPV Bacmids
- Improvement of GFPuv-β3GnT2 Fusion Protein Production by Suppressing Protease in Baculovirus Expression System(Biochemistry & Molecular Biology)
- The improvement of riboflavin production in Ashbya gossypii via disparity mutagenesis and DNA microarray analysis
- Bio-conversion of paper sludge to biofuel by simultaneous saccharification and fermentation using a cellulase of paper sludge origin and thermotolerant Saccharomyces cerevisiae TJ14
- 2Ap15 Bio-conversion of paper sludge to biofuel by simultaneous saccharification and fermentation using cellulase of paper sludge origin and thermotolerant Saccharomyces cerevisiae TJ14
- One-pot bioethanol production from cellulose by co-culture of Acremonium cellulolyticus and Saccharomyces cerevisiae
- Display of the human (pro)renin receptor on Bombyx mori nucleopolyhedrovirus (BmNPV) particles using Bm cells
- Display of the human (pro)renin receptor on Bombyx mori nucleopolyhedrovirus (BmNPV) particles using Bm cells(MEDICAL BIOTECHNOLOGY)