Mycelial Pellet Intrastructure Visualization and Viability Prediction in a Culture of Streptomycese fradiae Using Confocal Scanning Laser Microscopy

概要

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The intrastructure of mycelial pellets of Streptomyces fradiae, which produces tylosin, was visualized following labeling with fluorescein isothiocyanate (FITC) and propidium iodide (PI) using confocal scanning laser microscopy. A PI-labeled inactive core was present inside the mycelial pellet in both fermentor and air-lift reactor cultures. The thickness of the active mycelial layer on the pellet surface was calculated from a density sliced image to be 60 and 68 μm respectively, in the fermentor and in air-lift reactor cultures. Using image analysis, the active mycelial concentraion of pellets in the fermentor culture was predicted to be 2.1 times higher than that in the air-lift reactor culture. The tylosin production rate in the fermentor reached 0.78 g/l/d, which was 2.5-fold fhat in the air-lift reactor culture. These results indicate that the higher tylosin production rate in the fermentor culture was due to the higher active mycelial concentration in the fermentor compared to that in the air-lift reactor.
社団法人日本生物工学会の論文
1997-11-25